| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87283-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30452-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89446-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24592-25ul | |||
| Azide and BSA Free | NBP3-43860 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41152-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61844-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52436-0.025mg | |||
![Western Blot: Prohibitin Antibody [NBP1-87283] - Analysis in human cell lines Caco-2 and HeLa using Anti-PHB antibody. Corresponding PHB RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP1-87283-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Prohibitin Antibody [NBP1-87283] - Analysis in human cell lines Caco-2 and HeLa using Anti-PHB antibody. Corresponding PHB RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Prohibitin Antibody [NBP1-87283] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87283-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin Antibody [NBP1-87283] - Staining of human stomach shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Immunohistochemistry-Paraffin-NBP1-87283-img0015.jpg)
![Western Blot: Prohibitin Antibody [NBP1-87283] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Prohibitin-Antibody-Western-Blot-NBP1-87283-img0008.jpg)
![Western Blot: KATNAL2 Antibody [NBP2-30452] - Analysis in human cell lines A-549 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/KATNAL2-Antibody-Western-Blot-NBP2-30452-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: KATNAL2 Antibody [NBP2-30452] - Analysis in human cell lines A-549 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: KATNAL2 Antibody [NBP2-30452] - Staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.](http://images.novusbio.com/fullsize/KATNAL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30452-img0004.jpg)
![Immunohistochemistry-Paraffin: KATNAL2 Antibody [NBP2-30452] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/KATNAL2-Antibody-Immunohistochemistry-Paraffin-NBP2-30452-img0010.jpg)
![Immunohistochemistry-Paraffin: KATNAL2 Antibody [NBP2-30452] - Staining of human testis shows strong positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/KATNAL2-Antibody-Immunohistochemistry-Paraffin-NBP2-30452-img0005.jpg)
![Immunohistochemistry-Paraffin: KATNAL2 Antibody [NBP2-30452] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/KATNAL2-Antibody-Immunohistochemistry-Paraffin-NBP2-30452-img0007.jpg)
![Immunohistochemistry-Paraffin: KATNAL2 Antibody [NBP2-30452] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/KATNAL2-Antibody-Immunohistochemistry-Paraffin-NBP2-30452-img0008.jpg)
![Immunohistochemistry-Paraffin: KATNAL2 Antibody [NBP2-30452] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/KATNAL2-Antibody-Immunohistochemistry-Paraffin-NBP2-30452-img0009.jpg)
![Immunohistochemistry-Paraffin: KIF2B Antibody [NBP1-89446] - Staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/KIF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-89446-img0005.jpg)
![Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Western-Blot-NB100-74526-img0005.jpg "Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Analysis of purified RPA protein")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0013.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human kidney tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (MA34) [NB100-74526] - Analysis of RPA34 in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunocytochemistry-Immunofluorescence-NB100-74526-img0012.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0010.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (MA34) [NB100-74526] - Normal biopsies of deparaffinized Human tonsil tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/RPA2-Antibody-MA34-Immunohistochemistry-Paraffin-NB100-74526-img0014.jpg)
![Western Blot: LZTS2 Antibody [NBP2-41152] - Analysis of LZTS2 in human kidney tissue lysate with LZTS2 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LZTS2-Antibody-Western-Blot-NBP2-41152-img0001.jpg "Western Blot: LZTS2 Antibody [NBP2-41152] - Analysis of LZTS2 in human kidney tissue lysate with LZTS2 antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: LZTS2 Antibody [NBP2-41152] - Human kidney tissue with LZTS2 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/LZTS2-Antibody-Immunohistochemistry-Paraffin-NBP2-41152-img0002.jpg)
![Immunohistochemistry: LZTS2 Antibody [NBP2-41152] - Human kidney tissue with LZTS2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LZTS2-Antibody-Immunofluorescence-NBP2-41152-img0003.jpg)
![Immunohistochemistry: LZTS2 Antibody - BSA Free [NBP2-41152] - Immunohistochemistry of LZTS2 in human kidney tissue with LZTS2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41152_rabbit-polyclonal-lzts2-antibody-271120242040559.jpg)
![Immunocytochemistry/ Immunofluorescence: LZTS2 Antibody - BSA Free [NBP2-41152] - Immunofluorescence of LZTS2 in human kidney tissue with LZTS2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41152_rabbit-polyclonal-lzts2-antibody-271120241958457.jpg)
![Immunocytochemistry/ Immunofluorescence: LZTS2 Antibody - BSA Free [NBP2-41152] - Immunofluorescence of LZTS2 in human kidney tissue with LZTS2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41152_rabbit-polyclonal-lzts2-antibody-2711202420141942.jpg)
![Immunohistochemistry: LZTS2 Antibody - BSA Free [NBP2-41152] - Immunohistochemistry of LZTS2 in human kidney tissue with LZTS2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41152_rabbit-polyclonal-lzts2-antibody-2711202420195331.jpg)
![Western Blot: AIF Antibody [NBP2-42509] - Staining of Jurkat lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42509-img0003.jpg "Western Blot: AIF Antibody [NBP2-42509] - Staining of Jurkat lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42509-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42509-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42509] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42509-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42509] - Staining of paraffin embedded Human Kidney with antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42509-img0005.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0006.jpg "Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against HEK293 (1) and ALDOA (AA: 9-145)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of breast cancer tissues using ALDOA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Immunohistochemistry-NBP2-61844-img0004.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mAb against human ALDOA (AA: 9-145) recombinant protein. (Expected MW is 40.7 kDa)](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0005.jpg)
![Western Blot: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis using ALDOA mouse mAb against MCF-7 (1), Hela (2), and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Western-Blot-NBP2-61844-img0007.jpg)
![Immunohistochemistry-Paraffin: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of bladder cancer tissues using ALDOA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-Immunohistochemistry-NBP2-61844-img0003.jpg)
![ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-ELISA-NBP2-61844-img0001.jpg)
![ELISA: Aldolase A Antibody (1C5B2) [NBP2-61844] - Analysis of K562 cells using ALDOA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Aldolase-A-Antibody-1C5B2-ELISA-NBP2-61844-img0002.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0007.jpg "Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.")
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0003.jpg)
![Flow Cytometry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Flow-Cytometry-NBP2-52436-img0001.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0005.jpg)
![Western Blot: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Western-Blot-NBP2-52436-img0004.jpg)
![Immunohistochemistry: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-Immunohistochemistry-NBP2-52436-img0002.jpg)
![ELISA: Cyclin A2 Antibody (6B4D11) [NBP2-52436] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-6B4D11-ELISA-NBP2-52436-img0006.jpg)
![Western Blot: HSP27 Antibody (5D7) [NBP2-37599] - Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Western-Blot-NBP2-37599-img0002.jpg "Western Blot: HSP27 Antibody (5D7) [NBP2-37599] - Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (5D7) [NBP2-37599] - Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Immunocytochemistry-Immunofluorescence-NBP2-37599-img0003.jpg)
![Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Immunohistochemistry-NBP2-37599-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (5D7) [NBP2-37599] - Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Flow-Cytometry-NBP2-37599-img0004.jpg)
![Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Immunohistochemistry-NBP2-37599-img0005.jpg)
![ELISA: HSP27 Antibody (5D7) [NBP2-37599] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-ELISA-NBP2-37599-img0001.jpg)
![Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Western-Blot-NBP1-97687-img0003.jpg "Western Blot: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunoblotting result of NBP1-97687(131C3) recognizing nuclear lamins A and C in human fibroblasts.")
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry on frozen tissue section of human skin showing nuclear lamina staining in epithelial and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0004.jpg)
![Immunohistochemistry: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of on frozen sections of swine skin showing strong positive staining in nuclei of the epidermal cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-NBP1-97687-img0001.jpg)
![Immunohistochemistry-Frozen: Lamin A + C Antibody (131C3) [NBP1-97687] - Immunohistochemistry of NBP1-97687(131C3) on frozen sections of human colon showing strong positive staining in nuclei of the epithelial cells and to a lesser extent in the connective tissue.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-131C3-Immunohistochemistry-Frozen-NBP1-97687-img0002.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0004.jpg "Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Immunofluorescence-NBP2-54621-img0006.jpg)
![Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HDAC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54621-img0001.jpg)
![Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Western-Blot-NBP2-54621-img0003.jpg)
![ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.](http://images.novusbio.com/fullsize/HDAC1-Antibody-ELISA-NBP2-54621-img0002.jpg)
![Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.](http://images.novusbio.com/fullsize/HDAC1-Antibody-Protein-Array-NBP2-54621-img0005.jpg)