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![Western Blot: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: IdU. Lane 5 CldU. Load: 20 Ug per lane.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-29G6-E8-Western-Blot-NBP2-44055-img0001.jpg "Western Blot: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: IdU. Lane 5 CldU. Load: 20 Ug per lane.")
![Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing hindbrain. Fixation: 4% PFA. Antigen retrieval: not required. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-29G6-E8-Immunocytochemistry-Immunofluorescence-NBP2-44055-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-29G6-E8-Immunocytochemistry-Immunofluorescence-NBP2-44055-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 40X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4C. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-29G6-E8-Immunocytochemistry-Immunofluorescence-NBP2-44055-img0007.jpg)
![Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-29G6-E8-N-A-NBP2-44055-img0009.jpg)
![Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CtIP-Antibody-Immunoprecipitation-NB100-79809-img0003.jpg "Immunoprecipitation: CtIP Antibody [NB100-79809] - Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CtIP antibody BL1913 (NB100-79809) used at 3 ug/mg lysate for IP. CtIP was also immunoprecipitated with rabbit anti-CtIP antibody BL1914 (NB100-79810) using 3 ug/mg lysate. Immunoprecipitated CtIP was blotted using BL1914 at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NB100-79808-img0014.jpg "Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NB100-79808-img0012.jpg)
![Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Western-Blot-NB100-79808-img0013.jpg)
![Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbitanti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-Paraffin-NB100-79808-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody NB100-79808 (lot NB100-79808-2) used for IP at 6 ug per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79808-1) and rabbit anti-RCH1/KPNA2 antibody NB100-79807. For blotting immunoprecipitated RCH1/KPNA2, NB100-79808 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79808-img0010.jpg)
![Western Blot: LATS1 Antibody [NB200-197] - Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 ug) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody NB200-197 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/LATS1-Antibody-Western-Blot-NB200-197-img0002.jpg "Western Blot: LATS1 Antibody [NB200-197] - Detection of mouse LATS1 by western blot. Samples: Whole cell lysate (50 ug) from CT26.WT and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-LATS1 antibody NB200-197 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: LATS1 Antibody [NB200-197] - Detection of Human LATS1 on HeLa whole cell lysate using NB200-197. LATS1 was also immunoprecipitated with another LATS1 antibody and NB200-198 using 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/LATS1-Antibody-Western-Blot-NB200-197-img0001.jpg)
![Western Blot: PP2A alpha Antibody [NBP2-19896] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NBP2-19896-img0005.jpg "Western Blot: PP2A alpha Antibody [NBP2-19896] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PP2A alpha Antibody [NBP2-19896] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using PP2A alpha antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19896-img0004.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NBP2-19896] - PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PP2A alpha stained by PP2A alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19896-img0007.jpg)
![Western Blot: PP2A alpha Antibody [NBP2-19896] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Western-Blot-NBP2-19896-img0002.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NBP2-19896] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19896-img0003.jpg)
![Immunohistochemistry-Paraffin: PP2A alpha Antibody [NBP2-19896] - PP2A alpha antibody detects PP2A alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. PP2A alpha stained by PP2A alpha antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PP2A-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-19896-img0006.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0009.jpg "Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-33558] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunohistochemistry-Paraffin-NBP1-33558-img0003.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0004.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0005.jpg)
![Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bub3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Bub3-Antibody-Western-Blot-NBP1-33558-img0008.jpg)
![Immunoprecipitation: Bub3 Antibody [NBP1-33558] - Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 ug of BUB3 antibody [N1C1]. Western blot analysis was performed using BUB3 antibody [N1C1]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Bub3-Antibody-Immunoprecipitation-NBP1-33558-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SESN3 Antibody [NBP1-31702] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SESN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31702-img0002.jpg "Immunocytochemistry/Immunofluorescence: SESN3 Antibody [NBP1-31702] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.")
![Western Blot: SESN3 Antibody [NBP1-31702] - A. 30 ug Rat 2 whole cell lysate/extract 7.5 % SDS-PAGESESN3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/SESN3-Antibody-Western-Blot-NBP1-31702-img0005.jpg)
![Western Blot: SESN3 Antibody [NBP1-31702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/SESN3-Antibody-Western-Blot-NBP1-31702-img0007.jpg)
![Western Blot: SESN3 Antibody [NBP1-31702] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31702_rabbit-polyclonal-sesn3-antibody-western-blot-202202493440..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SESN3 Antibody [NBP1-31702] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31702_rabbit-polyclonal-sesn3-antibody-1610202419405757.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Western-Blot-NBP1-31325-img0005.jpg "Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.")
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Mouse colon. 14-3-3 zeta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-31325-img0006.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-zeta-Antibody-Immunohistochemistry-Paraffin-NBP1-31325-img0003.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31325_rabbit-polyclonal-14-3-3-zeta-antibody-91020242013121.jpg)
![Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31325_rabbit-polyclonal-14-3-3-zeta-antibody-91020242075967.jpg)
![Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.](http://images.novusbio.com/fullsize/HDAC7-[p-Ser318]-Antibody-Western-Blot-NBP2-03978-img0003.jpg "Western Blot: HDAC7 [p Ser318] Antibody [NBP2-03978] - Analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containinug an unphosphorylated serine at position 318, using HDAC7 phospho antibody at 0.1 ug/mL.")
![Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - beta-amyloid (pyroglu11) NBP1-44070 in red (left) co-labeled with 6E10 in green (middle) and merge (right). Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu11]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44070-img0003.jpg "Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - beta-amyloid (pyroglu11) NBP1-44070 in red (left) co-labeled with 6E10 in green (middle) and merge (right). Image from verified customer review.")
![Western Blot: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - Lane 1: Ovalbumin Control. Lane 2: beta Amyloid pyro E11 peptide. Lane 3: beta Amyloid 11 peptide. Lane 4: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: Beta Amyloid pyro E11 antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E11 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu11]-Antibody-Western-Blot-NBP1-44070-img0008.jpg)
![Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE11Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44070 antibody.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu11]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44070-img0005.jpg)
![Western Blot: N-Cadherin Antibody (8C11) [NBP2-53321] - Western blot analysis of Heart tissue lysate using N-Cadherin antibody (8C11).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-8C11-Western-Blot-NBP2-53321-img0002.jpg "Western Blot: N-Cadherin Antibody (8C11) [NBP2-53321] - Western blot analysis of Heart tissue lysate using N-Cadherin antibody (8C11).")
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (8C11) [NBP2-53321] - Mouse heart stained with N-Cadherein antibody (8C11).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-8C11-Immunohistochemistry-Paraffin-NBP2-53321-img0001.jpg)
![Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP2-17086] - Cal27 Xenograft, using SUV39H1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Immunohistochemistry-Paraffin-NBP2-17086-img0003.jpg "Immunohistochemistry-Paraffin: KMT1A/SUV39H1 Antibody [NBP2-17086] - Cal27 Xenograft, using SUV39H1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: KMT1A/SUV39H1 Antibody [NBP2-17086] - Western blot with anti Suv39H1 after siControl and siSUV39H1 in MCF7 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/KMT1A-SUV39H1-Antibody-Western-Blot-NBP2-17086-img0005.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUV39H1 antibody [N3C3] (NBP2-17086) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17086_rabbit-polyclonal-kmt1a-suv39h1-antibody-western-blot-26112025199121.jpg)
![SUV39H1 antibody [N3C3] detects SUV39H1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SUV39H1 stained by SUV39H1 antibody [N3C3] (NBP2-17086) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-17086_rabbit-polyclonal-kmt1a-suv39h1-antibody-immunocytochemistry-immunofluorescence-2611202518404821.jpg)