| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-212SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-2860-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73253 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71662SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0003.jpg "Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody (4E7) [NBP2-37577] - Analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NBP2-37577-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human lung cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0009.jpg)
![Flow Cytometry: AIF Antibody (4E7) [NBP2-37577] - Analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Flow-Cytometry-NBP2-37577-img0005.jpg)
![Western Blot: AIF Antibody (4E7) [NBP2-37577] - Analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Western-Blot-NBP2-37577-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human breast cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human cervical cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody (4E7) [NBP2-37577] - Analysis of human rectum cancer tissues using AIF mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-Immunohistochemistry-NBP2-37577-img0008.jpg)
![ELISA: AIF Antibody (4E7) [NBP2-37577] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/AIF-Antibody-4E7-ELISA-NBP2-37577-img0001.jpg)
![Western Blot: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-SS-53-Western-Blot-NB500-212-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.")
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0012.jpg "Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 monoclonal antibody (M01), clone 1E1. Analysis of RRM2 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunocytochemistry-Immunofluorescence-H00006241-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of monoclonal antibody to RRM2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunohistochemistry-Paraffin-H00006241-M01-img0009.jpg)
![Western Blot: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Western-Blot-H00006241-M01-img0002.jpg)
![Immunoprecipitation: RRM2 Antibody (1E1) [H00006241-M01] - Analysis of RRM2 transfected lysate using anti-RRM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RRM2 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-Immunoprecipitation-H00006241-M01-img0010.jpg)
![ELISA: RRM2 Antibody (1E1) [H00006241-M01] - RRM2 Antibody (1E1) [H00006241-M01] - Detection limit for recombinant GST tagged RRM2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RRM2-Antibody-1E1-ELISA-H00006241-M01-img0013.jpg)
![Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Western-Blot-NB120-2860-img0028.jpg "Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0008.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0013.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in MCF-7 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0017.jpg)
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat cerebellum tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0012.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in C6 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0018.jpg)
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in PC12 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0019.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Knockdown-Validated-H00005997-M01-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected and cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01] - Analysis of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Immunohistochemistry-Paraffin-H00005997-M01-img0005.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - RGS2 monoclonal antibody (M01), clone 4C4 Analysis of RGS2 expression in MCF-7.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-Western-Blot-H00005997-M01-img0007.jpg)
![ELISA: RGS2 Antibody (4C4) [H00005997-M01] - Detection limit for recombinant GST tagged RGS2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RGS2-Antibody-4C4-ELISA-H00005997-M01-img0008.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415394536.jpg)
![Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) & Western blotting (WB). A. Detection by RPPA of VCL & ERK2 in serial dilution of HEK293 cells transfected with siRNA targeting VCL (siVCL) or non-silencing siRNA control (siCTR). B. Linear regression was performed for ERK2 & VCL protein & Pearson correlation between dilution & the signal intensity was calculated for each condition of Figure 3A, with all p-values lower than 0.001. Signal intensities are expressed as mean of the replicates. C. Level of expression by WB & RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB & spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected & cropped by CiteAb from the following publication (//rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005997-m01_mouse-monoclonal-rgs2-antibody-4c4-310202415482256.jpg)
![Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Western-Blot-NBP1-47945-img0023.jpg "Western Blot: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Patched 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Patched 1.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody (OTI5C7) [NBP1-47945] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Patched 1.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunocytochemistry-NBP1-47945-img0013.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Human endometrium tissue.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0024.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0014.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0015.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0016.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0017.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0018.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0019.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Patched 1 Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0020.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human Kidney tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0021.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Human pancreas tissue using anti-Patched 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-5c7-Immunohistochemistry-Paraffin-NBP1-47945-img0022.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched 1-PTCH Antibody - BSA Free-Western Blot-NB200-118-img0004.jpg "Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. (C) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Patched 1/PTCH Antibody (NB200-118); Sonic Hedgehog/Shh Antibody (NBP2-22126). Magnification: 400x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB200-118-img0003.jpg)
![Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias. Immunostaining for Ptch1, showing increased protein levels throughout the growth plate in mutants, particularly in the resting zone. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1006352) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Immunohistochemistry-NB200-118-img0002.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Western-Blot-NB200-118-img0001.jpg)
![Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/RBBP4-RbAp48-Antibody-13D10-Immunoprecipitation-NBP1-41201-img0017.jpg "Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-western-blot-196202494114..jpg)
![RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] (NBP1-41201) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41201_mouse-monoclonal-rbbp4-rbap48-antibody-13d10-immunocytochemistry-immunofluorescence-2611202518283514.jpg)
![Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88345-img0017.jpg "Immunocytochemistry/Immunofluorescence: NCAPH Antibody [NBP1-88345] - Staining of human cell line U-2 OS shows localization to nucleus and cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining in human testis and kidney tissues . Corresponding NCAPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0016.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0014.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88345 (A) shows similar protein distribution across tissues to independent antibody NBP1-88346 (B).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human skin shows nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0013.jpg)
![Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human tonsil shows nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Immunohistochemistry-Paraffin-NBP1-88345-img0015.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Simple Western lane view shows a specific band for NCAPH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0006.jpg)
![Simple Western: NCAPH Antibody [NBP1-88345] - Electropherogram image(s) of corresponding Simple Western lane view. NCAPH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/NCAPH-Antibody-Simple-Western-NBP1-88345-img0007.jpg)
![Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Western-Blot-NBP1-71662-img0006.jpg "Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.")
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0005.jpg)
![Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a specific membrane-cytoplasmic staining in bronchiolar and alveolar epithelial cells with stronger signal towards the membranes of the cells.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody-Immunohistochemistry-Paraffin-NBP1-71662-img0004.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE3Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44048 antibody.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44048-img0005.jpg "Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE3Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44048 antibody.")
![Western Blot: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Lane 1-10: beta Amyloid pyro E3 peptide. Load: 1 ug per lane 1-2, 0.2 ug per lane 3-4, 0.05 ug per lane 5-6, 0.01 ug per lane 7-8, 0.005 ug per lane 9-10. Primary antibody: Beta Amyloid pyro E3 antibody at 0.5 ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide. Cross reactivity with Beta Amyloid 3 peptide is >1000 fold below pyrE3 reactivity and contains no reactivity towards the Beta Amyloid 42 A beta peptide (data not shown).](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Western-Blot-NBP1-44048-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Tissue: human brain section. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: beta Amyloid pyro E3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: beta Amyloid pyro E3 is nuclear and cytoplasmic. Staining: Top: Beta Amyloid pyro E3 as as green fluorescent signal, Beta Amyloid 3 as yellow signal; and Bottom: Beta Amyloid 3 as red signal with co-incubation of Beta Amyloid pyro E3 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44048-img0007.jpg)
![Western Blot: beta Amyloid [Pyro Glu3] Antibody [NBP1-44048] - Lane 1: beta Amyloid 3 peptide Lane 2: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: Beta Amyloid pyro E3 antibody at 2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E3 peptide.](http://images.novusbio.com/fullsize/beta-Amyloid-[Pyro-Glu3]-Antibody-Western-Blot-NBP1-44048-img0010.jpg)