| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42210SS | |||
| Azide and BSA Free | NBP2-80739 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85729-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44243 | |||
| BSA Free | NBP2-61430-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37928-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79874 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18559-25ul | |||
| Azide and BSA Free | NBP3-44372 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90746-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80972-25ul | |||
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Western-Blot-NBP2-42210-img0001.jpg "Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position.")
![Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells while the tumor stroma was largely negative for the staining.](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Immunohistochemistry-Paraffin-NBP2-42210-img0003.jpg)
![Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [NBP2-42210] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Flow-Cytometry-NBP2-42210-img0002.jpg)
![Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Western-Blot-NBP1-25966-img0017.jpg "Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.")
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess blocking solution was removed and cells were incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.](http://images.novusbio.com/fullsize/Proteinase-3-Myeloblastin-PRTN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-25966-img0020.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85729-img0010.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0011.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0012.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0013.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0014.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0011.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebellum shows strong positivity in both neurons and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0007.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining in human lymph node and cerebral cortex tissues using anti-CCL21 antibody. Corresponding CCL21 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining in human lymph node and cerebral cortex tissues using anti-CCL21 antibody. Corresponding CCL21 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0010.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0004.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0005.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human cerebral cortex shows no cytoplasmic positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0008.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human placenta shows no cytoplasmic positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0009.jpg)
![Immunohistochemistry-Paraffin: CCL21/6Ckine Antibody [NBP2-37928] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CCL21-6Ckine-Antibody-Immunohistochemistry-Paraffin-NBP2-37928-img0011.jpg)
![Western Blot: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Western Blot Analysis of A431 cell lysate using NPM1 Antibody (NPM1/1902).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Western-Blot-NBP2-79739-img0004.jpg "Western Blot: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Western Blot Analysis of A431 cell lysate using NPM1 Antibody (NPM1/1902).")
![Immunocytochemistry/Immunofluorescence: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Immunofluorescence staining of HeLa cells using NPM1-Monospecific Mouse Monoclonal Antibody (NPM1/1902) followed by goat anti-mouse IgG-CF488 (green). Phalloidin counterstain.](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Immunocytochemistry-Immunofluorescence-NBP2-79739-img0005.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with NPM1 Antibody (NPM1/1902).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Immunohistochemistry-Paraffin-NBP2-79739-img0002.jpg)
![Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with NPM1 Antibody (NPM1/1902).](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Immunohistochemistry-Paraffin-NBP2-79739-img0001.jpg)
![Protein Array: NPM1 Antibody (NPM1/1902) [NBP2-79739] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NPM1 Antibody (NPM1/1902) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/NPM1-Antibody-NPM1-1902-Protein-Array-NBP2-79739-img0003.jpg)
![Western Blot: GSPT1 Antibody (CL13336) [NBP3-18559] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Western-Blot-NBP3-18559-img0008.jpg "Western Blot: GSPT1 Antibody (CL13336) [NBP3-18559] - Analysis in human cell line K562.")
![Immunocytochemistry/Immunofluorescence: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of A-431 cells using the Anti-GSPT1 monoclonal antibody, showing specific staining in cytosol in green. Nuclear probes are visualized in blue (where available).](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunocytochemistry-Immunofluorescence-NBP3-18559-img0009.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunohistochemistry-Paraffin-NBP3-18559-img0016.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunohistochemistry-Paraffin-NBP3-18559-img0010.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human cervical cancer shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunocytochemistry-Immunofluorescence-NBP3-18559-img0011.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human colorectal cancer (metastasis to ovary) shows strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunohistochemistry-Paraffin-NBP3-18559-img0012.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human colorectal cancer shows moderate to strong cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunohistochemistry-Paraffin-NBP3-18559-img0013.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunohistochemistry-Paraffin-NBP3-18559-img0014.jpg)
![Immunohistochemistry-Paraffin: GSPT1 Antibody (CL13336) [NBP3-18559] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GSPT1-Antibody-CL13336-Immunohistochemistry-Paraffin-NBP3-18559-img0015.jpg)
![Western Blot: MCMBP Antibody [NBP1-90746] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/MCMBP-Antibody-Western-Blot-NBP1-90746-img0007.jpg "Western Blot: MCMBP Antibody [NBP1-90746] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: MCMBP Antibody [NBP1-90746] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MCMBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90746-img0009.jpg)
![Western Blot: MCMBP Antibody [NBP1-90746] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/MCMBP-Antibody-Western-Blot-NBP1-90746-img0005.jpg)
![Western Blot: SPARC Antibody [NBP1-80972] - Analysis in human cell lines U2OS and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SPARC Antibody [NBP1-80972] - Analysis in human cell lines U2OS and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: SPARC Antibody [NBP1-80972] - Analysis using Anti-SPARC antibody NBP1-80972 (A) shows similar pattern to independent antibody NBP1-80971 (B).](http://images.novusbio.com/fullsize/SPARC-Antibody-Western-Blot-NBP1-80972-img0013.jpg)
![Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80972] - Staining of human placenta shows strong positivity in endothelial cells and fibroblasts.](http://images.novusbio.com/fullsize/SPARC-Antibody-Immunohistochemistry-Paraffin-NBP1-80972-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP1-84814] - Staining of human cell line A-431 shows positivity in plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84814-img0006.jpg "Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP1-84814] - Staining of human cell line A-431 shows positivity in plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0017.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0015.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84814] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84814-img0016.jpg)
![Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Western-Blot-NBP2-37816-img0014.jpg "Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin in SHSY5Y cells (human neuroblast) either left untreated (left panel) or treated with 10uM retinoic acid (right panel) for 72 hours. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a beta-3 Tubulin monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37816-img0011.jpg)
![Immunohistochemistry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Normal biopsies of deparaffinized human brain tissues.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunohistochemistry-NBP2-37816-img0012.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody was performed and 10,000 cells were acquired for each sample.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Flow-Cytometry-NBP2-37816-img0010.jpg)
![Immunoprecipitation: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300ug of lysate with 5ug of a beta-3 Tubulin monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. SHSY5Y cell lysate (30ug) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunoprecipitation-NBP2-37816-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19324-img0020.jpg "Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody [NBP2-19324] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Lamin A + C stained by Lamin A + C antibody diluted at 1:1000. Red: alpha Tubulin, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mouse stomach. Lamin A + C stained by Lamin A + C antibody diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0022.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - C2C12 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - RT2 xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0005.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Human breast carcinoma. Lamin A + C antibody diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0007.jpg)
![Immunohistochemistry-Paraffin: Lamin A + C Antibody [NBP2-19324] - Mahlarvu xenograft, using Lamin A + C antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-Immunohistochemistry-Paraffin-NBP2-19324-img0018.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Lamin A + C Antibody [NBP2-19324] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-1610202419414527.jpg)
![Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.IP samples: HeLa whole cell extractA. 50 ug HeLa whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of Lamin A + C protein by 4 ug Lamin A + C antibody (NBP2-19324)7.5 % SDS-PAGEThe immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (NBP2-19324) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-19324_rabbit-polyclonal-lamin-a-c-antibody-immunoprecipitation-26112025199120.jpg)
