GSPT1 Antibody (CL13336) - Azide and BSA Free

Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.

Staining of A-431 cells using the Anti-GSPT1 monoclonal antibody) .

Staining of human colorectal cancer shows moderate to strong cytoplasmic positivity in tumor cells.

Staining of human colorectal cancer (metastasis to ovary) shows strong cytoplasmic positivity in tumor cells.

Staining of human cervical cancer shows strong cytoplasmic positivity in tumor cells.

Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Staining of human liver shows no positivity in hepatocytes.

Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Analysis in human cell line K562.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL13336
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GSPT1 Antibody (CL13336) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GSPT1 Antibody (CL13336) - Azide and BSA Free (NBP3-18559) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P15170, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSPT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for GSPT1 Antibody (CL13336) - Azide and BSA Free

• ERF3A
• eRF3aFLJ38048
• ETF3A
• eukaryotic peptide chain release factor GTP-binding subunit ERF3A
• Eukaryotic peptide chain release factor subunit 3a
• FLJ39067
• G1 to S phase transition 1,551G9.2
• G1 to S phase transition protein 1 homolog
• GST1

Background

GSPT1 is involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates theactivity of ERF1. Involved in regulation of mammalian cell growth

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSPT1