| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44018 | |||
| BSA Free | NBP2-30699-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82648-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89517-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84976-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84322-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70733 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96431-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61764-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFIB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81000-img0009.jpg "Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.")
![Western Blot: NFIB Antibody [NBP1-81000] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/NFIB-Antibody-Western-Blot-NBP1-81000-img0007.jpg)
![Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Western-Blot-NBP2-30699-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dicer Antibody (CL0378) [NBP2-30699] - Staining of A-431 cells using the anti-DICER1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunocytochemistry-Immunofluorescence-NBP2-30699-img0018.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human breast cancer (ductal carcinoma) shows moderate cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0017.jpg)
![Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Lane 1: Marker [kDa]. Lane 2: Human cell line HEK293T](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Western-Blot-NBP2-30699-img0008.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0011.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human testis shows cytoplasmic positivity in the seminiferous tubules cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0012.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human prostate shows cytoplasmic immunoreactivity in the glandular epithelium cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0013.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human cerebral cortex shows cytoplasmic positivity in the glial cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0014.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human colorectal cancer (adenocarcinoma) shows cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0015.jpg)
![Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human lung cancer (adenocarcinoma) shows strong cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/Dicer-Antibody-CL0378-Immunohistochemistry-Paraffin-NBP2-30699-img0016.jpg)
![Western Blot: DHPS Antibody [NBP1-82648] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/DHPS-Antibody-Western-Blot-NBP1-82648-img0008.jpg "Western Blot: DHPS Antibody [NBP1-82648] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: DHPS Antibody [NBP1-82648] - Staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82648-img0007.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0012.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0009.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human fallopian tube shows moderate cytoplasmic/membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0010.jpg)
![Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82648] - Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/DHPS-Antibody-Immunohistochemistry-Paraffin-NBP1-82648-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-89517] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89517-img0007.jpg "Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-89517] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0021.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human cerebral cortex, fallopian tube, prostate and testis using Anti-NUDC antibody NBP1-89517 (A) shows similar protein distribution across tissues to independent antibody NBP1-89510 (B).](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0019.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human cerebral cortex shows weak to moderate cytoplasmic and nuclear positivity in neurons.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0018.jpg)
![Immunohistochemistry-Paraffin: NUDC Antibody [NBP1-89517] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NUDC-Antibody-Immunohistochemistry-Paraffin-NBP1-89517-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: NUDC Antibody [NBP1-89517] - Analysis using Anti-NUDC antibody NBP1-89517 (A) shows similar pattern to independent antibody NBP1-89510 (B).](http://images.novusbio.com/fullsize/NUDC-Antibody-Western-Blot-NBP1-89517-img0013.jpg)
![Western Blot: ERH Antibody [NBP1-84976] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ERH-Antibody-Western-Blot-NBP1-84976-img0007.jpg "Western Blot: ERH Antibody [NBP1-84976] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: ERH Antibody [NBP1-84976] - Staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.](http://images.novusbio.com/fullsize/ERH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84976-img0005.jpg)
![Western Blot: ERH Antibody [NBP1-84976] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/ERH-Antibody-Western-Blot-NBP1-84976-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NBP1-84322] - Staining of human cell line A549 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84322-img0010.jpg "Immunocytochemistry/Immunofluorescence: STIM1 Antibody [NBP1-84322] - Staining of human cell line A549 shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0021.jpg)
![Western Blot: STIM1 Antibody [NBP1-84322] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/STIM1-Antibody-Western-Blot-NBP1-84322-img0007.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0013.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts..](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0014.jpg)
![Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: STIM1 Antibody [NBP1-84322] - Staining of human cerebellum, fallopian tube, kidney and testis using Anti-STIM1 antibody NBP1-84322 (A) shows similar protein distribution across tissues to independent antibody NBP1-84323 (B).](http://images.novusbio.com/fullsize/STIM1-Antibody-Immunohistochemistry-Paraffin-NBP1-84322-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85638] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RCC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85638-img0011.jpg "Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85638] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0007.jpg "Western Blot: Fumarase Antibody (1F10) [NBP1-47754] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Fumarase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Fumarase Antibody (1F10) [NBP1-47754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunocytochemistry-Immunofluorescence-NBP1-47754-img0006.jpg)
![Immunohistochemistry: Fumarase Antibody (OTI1F10) [NBP1-47754] - Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human colon tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-OTI1F10-Immunohistochemistry-NBP1-47754-img0008.jpg)
![Flow Cytometry: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Fumarase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Flow-Cytometry-NBP1-47754-img0001.jpg)
![Western Blot: Fumarase Antibody (1F10) [NBP1-47754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fumarase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Fumarase.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Western-Blot-NBP1-47754-img0002.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human endometrium tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0004.jpg)
![Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Staining of paraffin-embedded Human Kidney tissue using anti-Fumarase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Fumarase-Antibody-1F10-Immunohistochemistry-Paraffin-NBP1-47754-img0005.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0009.jpg "Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0008.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42496] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42496-img0016.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0004.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0005.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0007.jpg)
![Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Knockout-Validated-NB110-96431-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:8,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NB110-96431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (8A7) [NB110-96431] - analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Load: 15 ug protein. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Western-Blot-NB110-96431-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (8A7) [NB110-96431] - analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 . Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Courtesy of: Mike Welsh, Umich.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunocytochemistry-Immunofluorescence-NB110-96431-img0007.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0016.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (8A7) [NB110-96431] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-Paraffin-NB110-96431-img0008.jpg)
![Immunohistochemistry: HSP27 Antibody (8A7) [NB110-96431] - Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (NB110-96431). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (NB110-96431) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP27-Antibody-8A7-Immunohistochemistry-NB110-96431-img0017.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0008.jpg "Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0006.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0007.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0003.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0005.jpg)
![ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0001.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Western-Blot-NBP1-51643-img0010.jpg "Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.")
![Immunohistochemistry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Images of IHC analysis of B7-H3 and HK2 protein expression and hematoxylin and eosin (H&E) staining of CRC (n=126) tissue sections. One representative image is shown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-NBP1-51643-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Flow-Cytometry-NBP1-51643-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-Paraffin-NBP1-51643-img0009.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 promoted the expression of HK2 in CRC cells.a, b The expression of glycolysis-related genes was detected by RT-qPCR in both B7-H3-overexpressing HCT116 (a) & RKO (b) cells. c, d The mRNA level of HK2 was detected by RT-qPCR in both HCT116 (c) & RKO (d) cells after transfection with siRNA NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. e HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both HCT116 & RKO cells after transfection with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. beta -actin served as a loading control. f HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both B7-H3-overexpressing HCT116 & RKO cells. beta -actin served as a loading control. g Schematic representation of the proposed B7-H3/STAT3/HK2 axis. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202416173538.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 regulated glycolysis through HK2.a The protein level of HK2 in B7-H3-overexpressing HCT116 or RKO cells after transfection with siRNA negative control (NC) or HK2 siRNA transfection were analyzed by western blot. beta -actin served as a loading control. b, c Glucose consumption (b) & lactate production (c) were measured in B7-H3-overexpressing HCT116 or RKO cells transfected with NC or HK2 siRNA. d, e Glucose consumption (d) & lactate production (e) were measured in B7-H3-overexpressing HCT116 or RKO cells treated with PBS or 2-DG. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202415175275.jpg)