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![Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - analysis of Human Cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 6C11-B3 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Western-Blot-NBP2-12882-img0003.jpg "Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - analysis of Human Cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 6C11-B3 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0007.jpg)
![Flow Cytometry: Cytokeratin 15 Antibody (LHK 15) [NBP2-50461] - An intracellular stain was performed on HeLa cells with Cytokeratin 15 Antibody (LHK 15) NBP2-50461PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-LHK-15-Flow-Cytometry-NBP2-50461-img0001.jpg "Flow Cytometry: Cytokeratin 15 Antibody (LHK 15) [NBP2-50461] - An intracellular stain was performed on HeLa cells with Cytokeratin 15 Antibody (LHK 15) NBP2-50461PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Western Blot: GSK-3 alpha/beta Antibody (1H8) [NB110-85523] - Analysis: Lane 1: MW marker, Lane 2: 20ug HeLa lysate (heat shocked), Lane 3: 20ug mouse brain lysate, Lane 4: 20ug rat brain lysate, Lane 5: 20ug RK-13 lysate.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-Antibody-1H8-Western-Blot-NB110-85523-img0003.jpg "Western Blot: GSK-3 alpha/beta Antibody (1H8) [NB110-85523] - Analysis: Lane 1: MW marker, Lane 2: 20ug HeLa lysate (heat shocked), Lane 3: 20ug mouse brain lysate, Lane 4: 20ug rat brain lysate, Lane 5: 20ug RK-13 lysate.")
![Immunohistochemistry-Paraffin: GSK3 alpha/beta Antibody (1H8) [NB110-85523] - Alzgeuner diseased neurons with granulovacuolar degeneration co-stained with GSK alpha/beta Monoclonal Antibody (1H8) (Brown Precipitate).](http://images.novusbio.com/fullsize/GSK3-alpha-beta-Antibody-1H8-Immunohistochemistry-Paraffin-NB110-85523-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139] - Formalin fixed paraffin embedded human skin stained with Cytokeratin 8/18 antibody (NB120-17139).](http://images.novusbio.com/fullsize/Cytokeratin-8-18-Antibody-5D3-Immunohistochemistry-Paraffin-NB120-17139-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139] - Formalin fixed paraffin embedded human skin stained with Cytokeratin 8/18 antibody (NB120-17139).")
![Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193] - Chicken gizzard cell extract was separated on SDS-PAGE and probed with NB120-11193. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/200.](http://images.novusbio.com/fullsize/Vinculin-Antibody-VIN-11-5-Western-Blot-NB120-11193-img0006.jpg "Western Blot: Vinculin Antibody (VIN-11-5) [NB120-11193] - Chicken gizzard cell extract was separated on SDS-PAGE and probed with NB120-11193. The antibody was developed with AlkPhos APA Mouse Fab ads HIgG and a NBT/BCIP substrate. Antibody dilution 1/200.")
![Immunocytochemistry/Immunofluorescence: Vinculin Antibody (VIN-11-5) [NB120-11193] - Double labeling of an isolated smooth muscle cell (chicken gizzard) with Monoclonal Anti-Vinculin, clone no. VIN-11-5 (green) and Anti-alpha Actinin (red).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VIN-11-5-Immunocytochemistry-Immunofluorescence-NB120-11193-img0005.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (C-542) [NBP1-19318] - Analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-C-542-Western-Blot-NBP1-19318-img0002.jpg "Western Blot: ER alpha/NR3A1 Antibody (C-542) [NBP1-19318] - Analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).")
![Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099] - Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:400 Monoclonal Anti-Nebulin antibody produced in Mouse, Clone: NB2. followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.](http://images.novusbio.com/fullsize/Nebulin-Antibody-NB2-Immunohistochemistry-NB120-11099-img0001.jpg "Immunohistochemistry: Nebulin Antibody (NB2) [NB120-11099] - Frozen sections, acetone fixed Chicken cardiac muscle were stained with 1:400 Monoclonal Anti-Nebulin antibody produced in Mouse, Clone: NB2. followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.")
![Immunohistochemistry-Paraffin: PAG1 Antibody (PAG-C1) [NB500-487] - staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.](http://images.novusbio.com/fullsize/PAG1-Antibody-PAG-C1-Immunohistochemistry-Paraffin-NB500-487-img0001.jpg "Immunohistochemistry-Paraffin: PAG1 Antibody (PAG-C1) [NB500-487] - staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.")
![Immunoprecipitation: PAG1 Antibody (PAG-C1) [NB500-487] - Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human Cbp/PAG (MEM-255). Note: Csk-binding protein (Cbp/PAG) is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-Cbp/PAG (PAG-C1) Lane 3: immunoprecipitation with anti-Cbp/PAG (polyclonal antibody).](http://images.novusbio.com/fullsize/PAG1-Antibody-PAG-C1-Immunoprecipitation-NB500-487-img0002.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0005.jpg "Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml")
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP2-87183] - Rabbit Anti-CHMP4B antibody. Formalin Fixed Paraffin Embedded Tissue: Human Kidney. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP2-87183-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Hela. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0002.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Mouse. Target Name: CHMP4B. Sample Tissue: Mouse Skeletal Muscle. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0003.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target: CHMP4B. Positive control (+): MCF7 (N10). Negative control (-): Human liver (LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0004.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0006.jpg)
![Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-7420252226526.png "Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or NBP3-43539(B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522141218.png)
![Western Blot: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Rat spleen (A), rat thymus (B) and rat lymph node (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-43539 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD74 is ~24.5-31.6kDa, depending on the isoform and post-translational modification. NBP3-43539 successfully detected CD74 in rat spleen, thymus and lymph node lysates.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522113030.png)