| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88706-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87490-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76639-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74375 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37507-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37345-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22191-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-21690-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97453-0.2mg | |||
| BSA Free | NBP1-97453-0.025mg | |||
![Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP3-11775-img0003.jpg "Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0008.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of mouse testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0002.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on Neuro2a cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0007.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0001.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on THP-1 cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SON Antibody [NBP1-88706] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SON-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88706-img0006.jpg "Immunocytochemistry/Immunofluorescence: SON Antibody [NBP1-88706] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human prostate shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0010.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human cerebellum shows moderate to strong positivity in nuclear speckles in Purkinje cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0008.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human small intestine shows moderate to strong positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0009.jpg)
![Immunohistochemistry-Paraffin: SON Antibody [NBP1-88706] - Staining of human tonsil shows moderate to strong positivity in nuclear speckles in germinal center cells.](http://images.novusbio.com/fullsize/SON-Antibody-Immunohistochemistry-Paraffin-NBP1-88706-img0011.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0008.jpg "Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Simple Western lane view shows a specific band for ARG1 in 0.2 mg/ml of Liver (left), HepG2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining in human liver and kidney tissues . Corresponding ARG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Analysis using Anti-ARG1 antibody NBP1-87490 (A) shows similar pattern to independent antibody NBP1-87455 (B).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Western-Blot-NBP1-87490-img0020.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in a subset of hematopoietic cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0015.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human hepatocellular carcinoma shows moderate cytoplasmic and nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0016.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0017.jpg)
![Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Immunohistochemistry-Paraffin-NBP1-87490-img0018.jpg)
![Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NBP1-87490] - Electropherogram image(s) of corresponding Simple Western lane view. Arginase 1/ARG1/liver Arginase antibody was used at 1:50 dilution on Liver and HepG2 lysate(s).](http://images.novusbio.com/fullsize/Arginase-1-ARG1-liver-Arginase-Antibody-Simple-Western-NBP1-87490-img0009.jpg)
![Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 ug of lysates per lane.Antibodies: NBP1-76654 (3 ug/mL), NBP1-76639 (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-271120242016915.gif)
![Immunocytochemistry/ Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in K562. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling PUMA with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-271120242045643.jpg)
![Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Carcinoma. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-2711202420243374.jpg)
![Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - Validation of PUMA in Human Breast Tissue. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PUMA antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76639_rabbit-polyclonal-puma-antibody-2711202420285891.gif)
![Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011] - Analysis of extracts (10ug) from 7 Human tissue by using anti-STAT1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.](http://images.novusbio.com/fullsize/STAT1-Antibody-OTI4H9-Western-Blot-NBP1-48011-img0009.jpg "Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011] - Analysis of extracts (10ug) from 7 Human tissue by using anti-STAT1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.")
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunocytochemistry-Immunofluorescence-NBP1-48011-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0006.jpg)
![Western Blot: STAT1 Antibody (4H9) [NBP1-48011] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Western-Blot-NBP1-48011-img0001.jpg)
![Western Blot: STAT1 Antibody (4H9) [NBP1-48011] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Western-Blot-NBP1-48011-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Immunofluorescent staining of HT29 cells using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunocytochemistry-Immunofluorescence-NBP1-48011-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human breast tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human endometrium tissue using anti-STAT1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT1-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48011-img0005.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0003.jpg "Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-37507-img0004.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of colon cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0007.jpg)
![Flow Cytometry: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of Jurkat cells using CDK2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Flow-Cytometry-NBP2-37507-img0005.jpg)
![Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Analysis using CDK2 mAb against human CDK2 (AA: 197-295) recombinant protein. (Expected MW is 36.8 kDa)](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Western-Blot-NBP2-37507-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of cervical cancer tissues using CDK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-NBP2-37507-img0006.jpg)
![Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - Analysis of CDK2 in colon cancer tissue using anti-CDK2 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-37507-img0008.jpg)
![ELISA: CDK2 Antibody (1A6) [NBP2-37507] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDK2-Antibody-1A6-ELISA-NBP2-37507-img0001.jpg)
![Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions: 1. Denatured, deglycosylated, reduced; 2. Denatured; 3. Denatured, reduced. Loading order: 1. Protein Ladder 2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced) 3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced) 4. Protein Ladder 5. HT1080 ALCAM-RFP (Denatured) 6. HT1080 ALCAM-KO (Denatured) 7. Protein Ladder 8. HT1080 ALCAM-RFP (Denatured, reduced) 9. HT1080 ALCAM-KO (Denatured, reduced). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-Western-Blot-NBP2-37345-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions: 1. Denatured, deglycosylated, reduced; 2. Denatured; 3. Denatured, reduced. Loading order: 1. Protein Ladder 2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced) 3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced) 4. Protein Ladder 5. HT1080 ALCAM-RFP (Denatured) 6. HT1080 ALCAM-KO (Denatured) 7. Protein Ladder 8. HT1080 ALCAM-RFP (Denatured, reduced) 9. HT1080 ALCAM-KO (Denatured, reduced). This image was submitted via customer Review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of prostate cancer tissues using ALCAM mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-Immunohistochemistry-Paraffin-NBP2-37345-img0008.jpg)
![Flow Cytometry: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Flow cytometric analysis of Jurkat cells using ALCAM mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-Flow-Cytometry-NBP2-37345-img0005.jpg)
![Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-Western-Blot-NBP2-37345-img0002.jpg)
![Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-Western-Blot-NBP2-37345-img0003.jpg)
![Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mouse mAb against NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-Western-Blot-NBP2-37345-img0004.jpg)
![Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of bladder cancer tissues using ALCAM mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-Immunohistochemistry-NBP2-37345-img0007.jpg)
![ELISA: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-4H9A5-ELISA-NBP2-37345-img0001.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0006.jpg "Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mouse mAb against Hela (1), HepG2 (2), A549 (3), Jurkat(4), L1210 (5) and Cos7 (6) cell lysate.")
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Flow cytometric analysis of Hela cells using Integrin beta 1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Flow-Cytometry-NBP2-22191-img0001.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Western blot analysis using Integrin beta 1 mAb against human ITGB1 (AA: 50-270) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Western-Blot-NBP2-22191-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Integrin beta 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22191-img0002.jpg)
![ELISA: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-ELISA-NBP2-22191-img0004.jpg)
![CyTOF-ready: Integrin beta 1/CD29 Antibody (3B6) [NBP2-22191] - Analysis of Integrin beta 1/CD29 antibody on human tissue. Integrin beta 1/CD29 (green). Scale, 50um. Image from verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-3B6-CyTOF-ready-NBP2-22191-img0007.jpg)
![Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5A5-Western-Blot-NBP1-21690-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - analysis of Rat skeletal muscle tissue lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5A5 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5A5) [NBP1-21690] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5A5-Immunocytochemistry-Immunofluorescence-NBP1-21690-img0005.jpg)
![Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Western-Blot-NB120-11003-img0004.jpg "Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.")
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-Actin produced in mouse clone No. AC-40. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - conserved C-terminus specific Monoclonal staining of human tongue frozen section with Monoclonal Anti-Actin](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:250 Monoclonal Anti-Actin Clone: AC-40. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody [NB600-579] - Human skin stained with Cytokeratin antibody.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-Immunohistochemistry-Paraffin-NB600-579-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody [NB600-579] - Human skin stained with Cytokeratin antibody.")
![Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of TNF-R1: Lane 1: MW marker, Lane 2: Hela (HS), Lane 3: Jurkat.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Western-Blot-NBP1-97453-img0005.jpg "Western Blot: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of TNF-R1: Lane 1: MW marker, Lane 2: Hela (HS), Lane 3: Jurkat.")
![Immunohistochemistry-Paraffin: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF-R1 Antibody [NBP1-97453] - Analysis of human spleen tissue stained with TNF receptor 1 pAb at 10ug/ml.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Immunohistochemistry-Paraffin-NBP1-97453-img0004.jpg)
![Flow Cytometry: TNF RI/TNFRSF1A Antibody [NBP1-97453] - TNF Receptor I Antibody [NBP1-97453] - MLL-AF9 transduced murine leukemia cells. Tnfr-/- cells were used as negative control. Image provided by verified customer.](http://images.novusbio.com/fullsize/TNF-RI-TNFRSF1A-Antibody-Flow-Cytometry-NBP1-97453-img0006.jpg)
![Western Blot: Rabbit Polyclonal TNF RI/TNFRSF1A Antibody [NBP1-97453] -Quantification of TNF alpha in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. NBP1-97453 was used at a dilution of 1:1000 and Beta-actin antibody (Catalog # NB600-501) was used at a dilution of 1:3000. Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 10 days after graft implantation. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97453_rabbit-polyclonal-tnf-ri-tnfrsf1a-antibody-western-blot-2952025141129.jpg)