| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52457-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86847-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56126SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75477SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-169SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
| IHC-Prediluted | NBP2-48351 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25200SS | |||
| Azide and BSA Free | NBP2-80635 | |||
![Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-[p-Ser40]-Antibody-NB300-173-img0005.jpg "Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [NB300-173] - Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-[p-Ser40]-Antibody-Immunohistochemistry-NB300-173-img0004.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0010.jpg "Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.")
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0006.jpg)
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0002.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0007.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0009.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0008.jpg)
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0005.jpg)
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0001.jpg)
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0003.jpg)
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0004.jpg)
![Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Western-Blot-NBP2-67667-img0008.jpg "Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0007.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0001.jpg)
![Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Frozen-NBP2-67667-img0004.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0003.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0005.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-SN06-42-Immunohistochemistry-Paraffin-NBP2-67667-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Monoclonal MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Human brain microvascular endothelial cells stained for ABCB1, red and ciliary MDR1/ABCB1 green and nuclear DAPI blue. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-67667_rabbit-monoclonal-mdr1-abcb1-antibody-sn06-42-immunocytochemistry-immunofluorescence-22202415364..png)
![Western Blot: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of MMP14 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Western-Blot-NBP2-67415-img0008.jpg "Western Blot: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of MMP14 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Immunocytochemistry-Immunofluorescence-NBP2-67415-img0006.jpg)
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Human endometrial adenocarcinoma paraffin-embedded tissue section. Tissue section was fixed, deparaffinized and EDTA was used for antigen retrieval. Incubated for 10 hours with primary antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Immunohistochemistry-Paraffin-NBP2-67415-img0010.jpg)
![Flow Cytometry: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Flow-Cytometry-NBP2-67415-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Immunocytochemistry-Immunofluorescence-NBP2-67415-img0007.jpg)
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Immunohistochemistry-Paraffin-NBP2-67415-img0002.jpg)
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Immunohistochemistry-Paraffin-NBP2-67415-img0003.jpg)
![Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MMP-14-MT1-MMP-Antibody-3-F7-Immunohistochemistry-Paraffin-NBP2-67415-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EMP2 Antibody [NBP1-86847] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86847-img0008.jpg "Immunocytochemistry/Immunofluorescence: EMP2 Antibody [NBP1-86847] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0015.jpg)
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human skin shows moderate to strong positivity in plasma membrane in keratinocytes.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0012.jpg)
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0013.jpg)
![Immunohistochemistry-Paraffin: EMP2 Antibody [NBP1-86847] - Staining of human lung shows moderate to strong cytoplasmic positivity in pneumocytes.](http://images.novusbio.com/fullsize/EMP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86847-img0016.jpg)
![Western Blot: EMP2 Antibody [NBP1-86847] - Analysis of EMP2 in NIH/3T3 transfectant using anti-EMP2 antibody (1:500 dilution). Image from verified customer review.](http://images.novusbio.com/fullsize/EMP2-Antibody-Western-Blot-NBP1-86847-img0005.jpg)
![Western Blot: Caspase-14 Antibody [NB100-56126] - Analysis of Caspase-14. Tissue lysates (50 ug/lane) and recombinant human Caspase-14 were (Hu C14, 15 ng) were western blotted with Caspase-14 antibody at 1:2000. The antibody detected both the proform of caspase-14, and the large and small subunits of active/cleaved caspase-14.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Western-Blot-NB100-56126-img0006.jpg "Western Blot: Caspase-14 Antibody [NB100-56126] - Analysis of Caspase-14. Tissue lysates (50 ug/lane) and recombinant human Caspase-14 were (Hu C14, 15 ng) were western blotted with Caspase-14 antibody at 1:2000. The antibody detected both the proform of caspase-14, and the large and small subunits of active/cleaved caspase-14.")
![Immunohistochemistry: Caspase-14 Antibody [NB100-56126] - Tissue sections of mouse skin at E17 stained using this antibody at 1:500.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Immunohistochemistry-NB100-56126-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Human ovarian cancer tissue microarray stained for Caspase-14 expression using this antibody at 1:2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1). Decreased Caspase-14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Immunohistochemistry-Paraffin-NB100-56126-img0004.jpg)
![Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Tissue sections of human cervix stained using this antibody at 1:2000. A. Normal cervix (squamous epithelium). B. CIN1 (low-grade squamous intraepithelial lesion, mild dysplasia). C. CIN2 (high-grade squamous intraepithelial lesion, moderate dysplasia. D. CIN3 (high-grade squamous intraepithelial lesion; severe dysplasia-carcinoma in situ. In normal cervi, caspase-14 staining was found most in the midzone layer, but was absent from the basal/parabasal cell layer where mitotically active cells are known to reside. This suggests induction of caspase-14 expression with differentiation. Caspase-14 expression declined progressively during malignant transformation as the histologic severity of the cervical atypia advanced from CIN1 to CIN3. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-14-Antibody-Immunohistochemistry-Paraffin-NB100-56126-img0005.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP1-75477-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-75477-img0006.jpg)
![Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-NBP1-75477-img0007.jpg)
![Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscle lysate.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP1-75477-img0008.jpg)
![Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Western-Blot-NB200-169-img0004.jpg "Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).")
![Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with NB200-169, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Immunocytochemistry-Immunofluorescence-NB200-169-img0003.jpg)
![Immunoprecipitation: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (Lanes 3), or NB200-169 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) from Arf null NIH3T3 cells (Lanes 1) and HA- p19Arf expressing cells (Lanes 2) were also analyzed by Western blotting, as input controls.](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Immunoprecipitation-NB200-169-img0005.jpg)
![Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-247-img0028.jpg "Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: Claspin Antibody [NB100-247] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Claspin-Antibody-Immunohistochemistry-NB100-247-img0027.jpg)
![Immunoprecipitation: Claspin Antibody [NB100-247] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Claspin,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Immunoprecipitation-NB100-247-img0023.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Immunohistochemistry-Paraffin-NBP2-44525-img0006.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).")
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Simple Western lane view shows a specific band for p21/CIP1/CDKN1A in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0002.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Electropherogram image of the corresponding Simple Western lane. p21/CIP1/CDKN1A antibody was used at 10 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0003.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720249362616.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Lane view shows a specific band for p21/CIP1/CDKN1A using 2 mg/mL of MCF-7 cell lysate and 10 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720241044618.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0013.jpg "Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - CD3+ T-Cells isolated from PBMCs stained with anti-CD2 Alexa Fluor 488 (BL1). Image from a verified customer review.")
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Cell surface analysis of CD2 in 10^6 human lymphocytes using 0.25 ug of CD2 RPA-2.10 antibody; Shaded histogram represents cells without antibody; green represents isotype control ; red represents CD2 antibody. Goat anti-mouse IgG PE conjugated secondary was used in this test.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0001.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25200. Staining of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD2.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0008.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Allophycocyanin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (open histogram) or Anti-Human CD2 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0009.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Biotin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.125 ug of Anti-Human CD2 Biotin (filled histogram) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0010.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the FITC conjugate of NBP2-25200. Staining of Human peripheral blood lymphocytes with RPA-2.10 FITC.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0011.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or Anti-Human CD2 PE-Cy5 (filled histogram). Total cells were used for analysis.](http://images.novusbio.com/fullsize/CD2-Antibody-RPA-2-10-Flow-Cytometry-NBP2-25200-img0012.jpg)