| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59311-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71540 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13336-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36770 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38199-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59378-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70150 | |||
![Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (NBP2-59311) at 1:1000.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Western-Blot-NBP2-59311-img0004.jpg "Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (NBP2-59311) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (NBP2-59311). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (NBP2-59311) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Immunocytochemistry-Immunofluorescence-NBP2-59311-img0005.jpg)
![Western Blot: SLC38A1 Antibody (S104-32) [NBP2-59311] - Analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32. Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Western-Blot-NBP2-59311-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SLC38A1 Antibody (S104-32) [NBP2-59311] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SNAT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SLC38A1-Antibody-S104-32-Immunocytochemistry-Immunofluorescence-NBP2-59311-img0003.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NB110-40560-img0005.jpg "Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of human LEDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF antibody NB110-40560 used for IP at 3 ug per reaction. LEDGF was also immunoprecipitated by rabbit anti-LEDGF antibody NB110-40561. For blotting immunoprecipitated LEDGF, NB110-40560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunoprecipitation-NB110-40560-img0006.jpg)
![Western Blot: ABCC5 Antibody (2C8) [NBP2-46467] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCC5.](http://images.novusbio.com/fullsize/ABCC5-Antibody-2C8-Western-Blot-NBP2-46467-img0006.jpg "Western Blot: ABCC5 Antibody (2C8) [NBP2-46467] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCC5.")
![Immunohistochemistry: ABCC5 Antibody (2C8) [NBP2-46467] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ABCC5-Antibody-2C8-Immunohistochemistry-NBP2-46467-img0005.jpg)
![Immunohistochemistry: ABCC5 Antibody (2C8) [NBP2-46467] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ABCC5-Antibody-2C8-Immunohistochemistry-NBP2-46467-img0001.jpg)
![Immunohistochemistry: ABCC5 Antibody (2C8) [NBP2-46467] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ABCC5-Antibody-2C8-Immunohistochemistry-NBP2-46467-img0002.jpg)
![Immunohistochemistry: ABCC5 Antibody (2C8) [NBP2-46467] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ABCC5-Antibody-2C8-Immunohistochemistry-NBP2-46467-img0003.jpg)
![Immunohistochemistry: ABCC5 Antibody (2C8) [NBP2-46467] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/ABCC5-Antibody-2C8-Immunohistochemistry-NBP2-46467-img0004.jpg)
![Western Blot: ABCF2 Antibody (1D11) [H00010061-M01] - ABCF2 monoclonal antibody (M01), clone 1D11 Analysis of ABCF2 expression in Hela.](http://images.novusbio.com/fullsize/ABCF2-Antibody-1D11-Western-Blot-H00010061-M01-img0012.jpg "Western Blot: ABCF2 Antibody (1D11) [H00010061-M01] - ABCF2 monoclonal antibody (M01), clone 1D11 Analysis of ABCF2 expression in Hela.")
![Immunohistochemistry-Paraffin: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of monoclonal antibody to ABCF2 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/ABCF2-Antibody-1D11-Immunohistochemistry-Paraffin-H00010061-M01-img0008.jpg)
![Western Blot: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of ABCF2 over-expressed 293 cell line, cotransfected with ABCF2 Validated Chimera RNAi ( Cat # H00010061-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ABCF2 monoclonal antibody (M01), clone 1D11 (Cat # H00010061-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/ABCF2-Antibody-1D11-Western-Blot-H00010061-M01-img0010.jpg)
![Western Blot: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of ABCF2 expression in transfected 293T cell line by ABCF2 monoclonal antibody (M01), clone 1D11.Lane 1: ABCF2 transfected lysate(68.53 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ABCF2-Antibody-1D11-Western-Blot-H00010061-M01-img0013.jpg)
![Immunoprecipitation: ABCF2 Antibody (1D11) [H00010061-M01] - Analysis of ABCF2 transfected lysate using anti-ABCF2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ABCF2 monoclonal antibody.](http://images.novusbio.com/fullsize/ABCF2-Antibody-1D11-Immunoprecipitation-H00010061-M01-img0009.jpg)
![Sandwich ELISA: ABCF2 Antibody (1D11) [H00010061-M01] - Detection limit for recombinant GST tagged ABCF2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ABCF2-Antibody-1D11-Sandwich-ELISA-H00010061-M01-img0011.jpg)
![Western Blot- SLC38A1 Antibody [NBP2-13336] -Analysis in human cell lines PC-3 and U2OS using Anti-SLC38A1 antibody. Corresponding SLC38A1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.](http://images.novusbio.com/fullsize/antibody/nbp2-13336_rabbit-polyclonal-slc38a1-antibody-western-blot-2710202312163..jpg "<b>Orthogonal Strategies Validation. </b>Western Blot- SLC38A1 Antibody [NBP2-13336] -Analysis in human cell lines PC-3 and U2OS using Anti-SLC38A1 antibody. Corresponding SLC38A1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLC38A1 Antibody [NBP2-13336] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in subset of neuronal cells.](http://images.novusbio.com/fullsize/antibody/nbp2-13336_rabbit-polyclonal-slc38a1-antibody-immunohistochemistry-paraffin-2710202312350..jpg)
![Immunohistochemistry-Paraffin: SLC38A1 Antibody [NBP2-13336] -Staining of human cerebellum shows moderate cytoplasmic positivity in subset of cells in granular layer.](http://images.novusbio.com/fullsize/antibody/nbp2-13336_rabbit-polyclonal-slc38a1-antibody-immunohistochemistry-paraffin-27102023123622..jpg)
![Immunohistochemistry-Paraffin: SLC38A1 Antibody [NBP2-13336] -Staining of human lymph node shows moderate membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp2-13336_rabbit-polyclonal-slc38a1-antibody-immunohistochemistry-paraffin-27102023123735..jpg)
![Immunohistochemistry-Paraffin: SLC38A1 Antibody [NBP2-13336] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-13336_rabbit-polyclonal-slc38a1-antibody-immunohistochemistry-paraffin-2710202312391..jpg)
![Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Western-Blot-NB110-40561-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.")
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody [NBP2-38199] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38199-img0004.jpg "Immunocytochemistry/Immunofluorescence: PMP70 Antibody [NBP2-38199] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining in human liver and skeletal muscle tissues using NBP2-38199 antibody. Corresponding ABCD3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0016.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human kidney, liver, skeletal muscle and testis using Anti-ABCD3 antibody NBP2-38199 (A) shows similar protein distribution across tissues to independent antibody NBP1-87258 (B).](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0012.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0013.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0014.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0015.jpg)
![Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Western-Blot-NBP2-59378-img0004.jpg "Western Blot: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Western Blot analysis of Rat brain membrane lysate showing detection of VAChT/SLC18A3 protein using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody, Clone S6-38 (NBP2-59378). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VAChT/SLC18A3 Monoclonal Antibody (NBP2-59378) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VAChT/SLC18A3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Immunocytochemistry-Immunofluorescence-NBP2-59378-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: VAChT/SLC18A3 Antibody (S6-38) [NBP2-59378] - Analysis using Mouse Anti-VAChT Monoclonal Antibody, Clone S6-38. Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VAChT Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-S6-38-Immunocytochemistry-Immunofluorescence-NBP2-59378-img0003.jpg)
![Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Knockdown-Validated-NBP2-46476-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Western-Blot-NBP2-46476-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0003.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0001.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0002.jpg)
![Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Western-Blot-NBP2-70150-img0001.jpg "Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.")
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0002.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Knockout-Validated-NBP2-70150-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)