| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39000-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87885-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43642-25ul | |||
| Azide and BSA Free | NBP3-44328 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14250-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44270-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13336-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59341-25ug | |||
![Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Western-Blot-NBP3-16348-img0003.jpg "Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Immunofluorescence analysis of NIH-3T3 cells using PMP70 Rabbit mAb (NBP3-16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Immunocytochemistry-Immunofluorescence-NBP3-16348-img0002.jpg)
![Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Western-Blot-NBP3-16348-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Immunofluorescence analysis of HeLa cells using PMP70 Rabbit mAb (NBP3-16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Immunocytochemistry-Immunofluorescence-NBP3-16348-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Confocal imaging of C2C12 cells using PMP70 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16348_rabbit-pmp70-mab-3v4k3-52202511402772.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Confocal imaging of HeLa cells using PMP70 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16348_rabbit-pmp70-mab-3v4k3-522025125489.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC17A7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC17A7 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebellum, cerebral cortex, pancreas and testis using Anti-VGLUT1 antibody NBP2-39000 (A) shows similar protein distribution across tissues to independent antibody NBP2-33597 (B).](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0011.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0013.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebral cortex shows positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0003.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0008.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0010.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87885-img0008.jpg "Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]-Staining of human breast shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp3-43642_mouse-abcb5-mab-cl13618-24120251545876.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA7 Antibody [NBP2-14250] - Staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14250-img0009.jpg "Immunocytochemistry/Immunofluorescence: ABCA7 Antibody [NBP2-14250] - Staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & cell junctions. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250] - Staining in human bone marrow and skeletal muscle tissues . Corresponding ABCA7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunohistochemistry-Paraffin-NBP2-14250-img0018.jpg)
![Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunohistochemistry-Paraffin-NBP2-14250-img0014.jpg)
![Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250] - Staining of human lymph node shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunohistochemistry-Paraffin-NBP2-14250-img0015.jpg)
![Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunohistochemistry-Paraffin-NBP2-14250-img0016.jpg)
![Immunohistochemistry-Paraffin: ABCA7 Antibody [NBP2-14250] - Staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp.](http://images.novusbio.com/fullsize/ABCA7-Antibody-Immunohistochemistry-Paraffin-NBP2-14250-img0017.jpg)
![Western Blot: ABCF2 Antibody [NBP1-89316] - Analysis using Anti-ABCF2 antibody NBP1-89316 (A) shows similar pattern to independent antibody NBP1-89317 (B).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP1-89316-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ABCF2 Antibody [NBP1-89316] - Analysis using Anti-ABCF2 antibody NBP1-89316 (A) shows similar pattern to independent antibody NBP1-89317 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89316] - Staining in human testis and liver tissues using anti-ABCF2 antibody. Corresponding ABCF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89316-img0008.jpg)
![Western Blot: ABCF2 Antibody [NBP1-89316] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ABCF2-Antibody-Western-Blot-NBP1-89316-img0005.jpg)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89316] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89316-img0006.jpg)
![Immunohistochemistry-Paraffin: ABCF2 Antibody [NBP1-89316] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ABCF2-Antibody-Immunohistochemistry-Paraffin-NBP1-89316-img0007.jpg)
![Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Western-Blot-NBP1-44270-img0009.jpg "Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.")
![Immunohistochemistry: SLC12A3 Antibody [NBP1-44270] - Immunohistochemistry analysis using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-NBP1-44270-img0010.jpg)
![Immunohistochemistry: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2/CD338 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31988, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-298202413585550.jpg "Immunohistochemistry: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2/CD338 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31988, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - ICC staining of ABCG2/CD338 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-31988, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-2920241033403.jpg)
![Flow Cytometry: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Flow cytometric analysis of ABCG2/CD338 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-31988, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-29202416372166.jpg)
![Western Blot: ABCG2/CD338 Antibody (JF0994) [NBP3-31988] - Western blot analysis of ABCG2/CD338 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-31988, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: human placenta tissue lysate Lane 3: Hela cell lysate Lane 4: 293T cell lysate](http://images.novusbio.com/fullsize/nbp3-31988_rabbit-abcg2-cd338-mab-jf0994-3920248453510.jpg)
![Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Western-Blot-NBP2-59341-img0009.jpg "Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000.")
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0012.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0010.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0011.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0013.jpg)