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ZNF667 Antibody - BSA Free

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Western Blot: ZNF667 Antibody [NBP1-77357] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.
Western Blot: ZNF667 Antibody [NBP1-77357] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: ZNF667 Antibody [NBP1-77357] - Immunofluorescence of MIPU in human brain tissue with MIPU antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: ZNF667 Antibody [NBP1-77357] - Human brain tissue with ZNF667 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-77357-0.025mg
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ZNF667 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human MIPU1. The immunogen is located within the first 50 amino acids of MIPU1. Amino Acid Squence: SQEEWEWLSPIQKDLYE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF667
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
ZNF667 Peptide (NBP1-77357PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for ZNF667 Antibody - BSA Free

  • DKFZp686O111
  • FLJ14011
  • FLJ45518
  • MIPU1
  • myocardial ischemic preconditioning upregulated 1 ortholog
  • zinc finger protein 667

Background

Mipu1, also known as zinc finger protein 667 or ZNF667, encodes a nuclear-localized protein containing 14 carboxy-terminal zinc finger motifs and an amino-terminal KRAB domain. This protein is highly expressed in heart and brain and is upregulated in rat heart after a transient ischemia-reperfusion procedure. Overexpression experiments suggest that Mipu1 suppresses the transcriptional activities of AP-1 and SRE in the MAPK signaling pathway and thus may play a role in the pathogenesis of cardiac and vascular disease. At least four isoforms of MIPU1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.
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Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
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Bioinformatics

Gene Symbol ZNF667
Uniprot