ZNF364 Antibody

Images

 
Western Blot: ZNF364 Antibody [NB100-41373] - (0.3ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ZNF364 Antibody Summary

Immunogen
Peptide with sequence C-DQDNRANERGHQTH corresponding to internal region according to NP_055270.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RNF115
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 35 kDa band observed in human HepG2 lysates (calculated MW of 33.7 kDa band according to NP_055270.1).
Positive Control
ZNF364 Lysate (NBL1-15414)
Publications
Read Publication using NB100-41373.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZNF364 Antibody

  • E3 ubiquitin-protein ligase RNF115
  • EC 6.3.2.-
  • Rabring 7
  • ring finger protein 115CL469780
  • Zinc finger protein 364BCA2
  • ZNF364

Background

The RING finger family of proteins possess ubiquitin ligase activity and play pivotal roles in protein degradation and receptor-mediated endocytosis. The autoubiquitination activity of ZNF364 indicates that it is a novel RING-type E3 ligase. Its effects on cell growth indicate that ZNF364 may be important for the ubiquitin modification of proteins crucial to breast carcinogenesis and growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZNF364 Antibody (NB100-41373)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNF115
Entrez
Uniprot