ZNF364 Antibody

Western Blot: ZNF364 Antibody [NB100-41373] - (0.3ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ZNF364 Antibody Summary

• Description: Novus Biologicals Goat ZNF364 Antibody (NB100-41373) is a polyclonal antibody validated for use in WB and ELISA. Anti-ZNF364 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DQDNRANERGHQTH corresponding to internal region according to NP_055270.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF115
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human HepG2 lysates (calculated MW of 33.7 kDa band according to NP_055270.1).
• Control:

  ZNF364 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF364 Antibody

• E3 ubiquitin-protein ligase RNF115
• EC 6.3.2.-
• Rabring 7
• ring finger protein 115CL469780
• Zinc finger protein 364BCA2
• ZNF364

Background

The RING finger family of proteins possess ubiquitin ligase activity and play pivotal roles in protein degradation and receptor-mediated endocytosis. The autoubiquitination activity of ZNF364 indicates that it is a novel RING-type E3 ligase. Its effects on cell growth indicate that ZNF364 may be important for the ubiquitin modification of proteins crucial to breast carcinogenesis and growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF364 Antibody (NB100-41373)(1)

Publication using NB100-41373

• Burger AM, Gao Y, Amemiya Y et al. A novel RING-type ubiquitin ligase breast cancer-associated gene 2 correlates with outcome in invasive breast cancer. Cancer Res 2005-11-15 [PMID: 16288031]

Bioinformatics

• Gene Symbol: RNF115
• Entrez:
  • 27246(Human)
  • 67845(Mouse)
  • 362002(Rat)
• Uniprot:
  • NP_055270.1(Human)