ZNF282 Antibody

Immunocytochemistry/Immunofluorescence: ZNF282 Antibody [NBP1-81653] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653] - Staining of human prostate shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: ZNF282 Antibody [NBP1-81653] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Product Discontinued

ZNF282 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DIAVYFSEDEWKNLDEWQKELYNNLVKENYKTLMSLDAEGSVPKPDAPVQAEPREEPCVWEQRHPEEREIPMDPEAGAEPLVPAQDASSQVKREDTLCVRGQRGLEERAIPTESITDSPISAQDLLSRIKQEEHQCVWDQQDLA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF282
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%). Reactivity reported in scientific literature (PMID: 25373738)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ZNF282 Antibody

• HTLV-I U5 repressive element-binding protein 1
• HTLV-I U5RE-binding protein 1
• HUB1
• zinc finger protein 282

Background

ZNF282 binds to the U5 repressive element (U5RE) of the human T cell leukemia virus type I long terminal repeat. It recognizes the 5'-TCCACCCC-3' sequence as a core motif and exerts a strong repressive effect on HTLV-I LTR-mediated expression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF282 Antibody (NBP1-81653)(1)

Publication using NBP1-81653

• Yeo SY, Ha SY, Yu EJ et al. ZNF282 (Zinc finger protein 282), a novel E2F1 co-activator, promotes esophageal squamous cell carcinoma. Oncotarget 2014-12-15 [PMID: 25373738] (IF/IHC, WB)

Bioinformatics

• Gene Symbol: ZNF282