ZNF10 Antibody (980809) [Unconjugated]

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, HeLa human cervical epithelial carcinoma cell line, human kidney tissue, and human thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ZNF10 Monoclonal Antibody (Catalog # MAB9656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ZNF10 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 980809
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ZNF10 Antibody (980809) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human ZNF10
  Accession # P21506
• Specificity: Detects human ZNF10 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZNF10 Antibody (980809) [Unconjugated]

• KOX1
• KOX1zinc finger protein 10 (KOX 1)
• zinc finger protein 10
• Zinc finger protein KOX1
• ZNF10

Background

Human Zinc finger protein ZNF10 (or KOX1) is a 573 amino acids protein encoded by the ZNF10 gene. ZNF10 contains a C2H2 zinc finger, and has been shown to function as a transcriptional repressor via the Kruppel-associated box (KRAB) domain of the protein. ZNF10 has been shown to interact with the nuclear hub protein TRIM28 to mediate chromatin-dependent processes like transcriptional repression, imprinting or suppression of endogenous retroviruses, representing a potential intrinsic antiretroviral defense.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P21506()