ZNF10 Antibody (980803) [Unconjugated]

ZNF10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human ZNF10 Monoclonal Antibody (Catalog # MAB96561) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 980803
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ZNF10 Antibody (980803) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human ZNF10
  Accession # P21506
• Specificity: Detects human ZNF10 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZNF10 Antibody (980803) [Unconjugated]

• KOX1
• KOX1zinc finger protein 10 (KOX 1)
• zinc finger protein 10
• Zinc finger protein KOX1
• ZNF10

Background

Human Zinc finger protein ZNF10 (or KOX1) is a 573 amino acids protein encoded by the ZNF10 gene. ZNF10 contains a C2H2 zinc finger, and has been shown to function as a transcriptional repressor via the Kruppel-associated box (KRAB) domain of the protein. ZNF10 has been shown to interact with the nuclear hub protein TRIM28 to mediate chromatin-dependent processes like transcriptional repression, imprinting or suppression of endogenous retroviruses, representing a potential intrinsic antiretroviral defense.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P21506()