ZIC4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ZIC4 Antibody [NBP2-37867] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.

Immunohistochemistry-Paraffin: ZIC4 Antibody [NBP2-37867] - Staining of human cerebral cortex shows nucleolar positivity in neuronal cells.

Product Discontinued

ZIC4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LVMRKRLRLYRNTLKESSSSSGHHGPQLTAASSPSVFPGLHEEPPQASPSRPLNGLLRLGLPGDMYARPEPFPPGPAARSDALAAAAALHGYGGMNLTVNL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZIC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ZIC4 Antibody - BSA Free

• FLJ42609
• FLJ45833
• Zic family member 4
• Zinc finger protein of the cerebellum 4zinc family member 4 protein HZIC4
• zinc finger protein ZIC 4

Background

ZIC4 encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. This gene encodes a protein of unknown function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZIC4
• Uniprot:
  • Q8N9L1(Human)