ZDHHC17 Antibody

Images

 
Western Blot: ZDHHC17 Antibody [NB100-1505] - (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ZDHHC17 Antibody [NB100-1505] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ZDHHC17 Antibody Summary

Immunogen
Peptide with sequence CYDQISGSGYQLV corresponding to C-Terminus according to NP_056151.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ZDHHC17
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 70 kDa band observed in human and mouse brain lysates (calculated MW of 72.7 kDa band according to NP_056151.1).
IHC: Paraffin embedded Human Testis.
Positive Control
ZDHHC17 Lysate (NBL1-18003)
Publications
Read Publication using NB100-1505.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZDHHC17 Antibody

  • DHHC-17
  • EC 2.3.1
  • EC 2.3.1.-
  • HIP14HIP-14
  • huntingtin interacting protein 14
  • huntingtin interacting protein 3
  • Huntingtin interacting protein H
  • Huntingtin yeast partner H
  • Huntingtin-interacting protein 14
  • Huntingtin-interacting protein 3
  • Huntingtin-interacting protein H
  • HYPHHIP-3
  • KIAA0946HIP3
  • palmitoyltransferase ZDHHC17
  • Putative MAPK-activating protein PM11
  • Putative NF-kappa-B-activating protein 205
  • Zinc finger DHHC domain-containing protein 17
  • zinc finger, DHHC domain containing 17
  • zinc finger, DHHC-type containing 17

Background

ZDHHC17 / HIP14 is a neuronal palmitoyl transferase. Palmitoylation is critical for trafficking and function of signaling molecules, neurotransmitter receptors, and synaptic scaffolding proteins in neurons. ZDHHC17 also causes cellular transformation. It has been shown that mRNA encoding ZDHHC17 is up-regulated in a number of types of human tumors, thus ZDHHC17 and other PATs (palmitoyl acyltransferases) are potential targets for new anticancer drugs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZDHHC17 Antibody (NB100-1505)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZDHHC17
Entrez
Uniprot