ZDHHC17 Antibody

Western Blot: ZDHHC17 Antibody [NB100-1505] - (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ZDHHC17 Antibody [NB100-1505] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ZDHHC17 Antibody Summary

• Description: Novus Biologicals Goat ZDHHC17 Antibody (NB100-1505) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ZDHHC17 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CYDQISGSGYQLV corresponding to C-Terminus according to NP_056151.2.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZDHHC17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human and mouse brain lysates (calculated MW of 72.7 kDa band according to NP_056151.1).
  IHC: Paraffin embedded Human Testis.
• Control:

  ZDHHC17 Overexpression Lysate

• Control Peptide:

  ZDHHC17 Protein (NB100-1505PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZDHHC17 Antibody

• DHHC-17
• EC 2.3.1
• EC 2.3.1.-
• HIP14HIP-14
• huntingtin interacting protein 14
• huntingtin interacting protein 3
• Huntingtin interacting protein H
• Huntingtin yeast partner H
• Huntingtin-interacting protein 14
• Huntingtin-interacting protein 3
• Huntingtin-interacting protein H
• HYPHHIP-3
• KIAA0946HIP3
• palmitoyltransferase ZDHHC17
• Putative MAPK-activating protein PM11
• Putative NF-kappa-B-activating protein 205
• Zinc finger DHHC domain-containing protein 17
• zinc finger, DHHC domain containing 17
• zinc finger, DHHC-type containing 17

Background

ZDHHC17 / HIP14 is a neuronal palmitoyl transferase. Palmitoylation is critical for trafficking and function of signaling molecules, neurotransmitter receptors, and synaptic scaffolding proteins in neurons. ZDHHC17 also causes cellular transformation. It has been shown that mRNA encoding ZDHHC17 is up-regulated in a number of types of human tumors, thus ZDHHC17 and other PATs (palmitoyl acyltransferases) are potential targets for new anticancer drugs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZDHHC17 Antibody (NB100-1505)(1)

Publication using NB100-1505

• Huang K, Yanai A, Kang R et al. Huntingtin-Interacting Protein HIP14 Is a Palmitoyl Transferase Involved in Palmitoylation and Trafficking of Multiple Neuronal Proteins. Neuron 2004-12-16 [PMID: 15603740]

Bioinformatics

• Gene Symbol: ZDHHC17
• Entrez:
  • 23390(Human)
• Uniprot:
  • NP_056151.(Human)