ZBTB48 Antibody

Western Blot: ZBTB48 Antibody [NBP2-75720] - Analysis of ZBTB48 on mouse spleen tissue lysate using anti-ZBTB48 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ZBTB48 Antibody [NBP2-75720] - Staining ZBTB48 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ZBTB48 Antibody [NBP2-75720] - Analysis of paraffin-embedded rat kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.

Flow Cytometry: ZBTB48 Antibody [NBP2-75720] - Analysis of Hela cells with ZBTB48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ZBTB48 Antibody [NBP2-75720] - Staining ZBTB48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ZBTB48 Antibody [NBP2-75720] - Analysis of paraffin-embedded human liver cancer tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ZBTB48 Antibody [NBP2-75720] - Analysis of paraffin-embedded human kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ZBTB48 Antibody [NBP2-75720] - Analysis of paraffin-embedded mouse kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin.

Product Discontinued

ZBTB48 Antibody Summary

• Immunogen: Synthetic peptide within Human ZBTB48 aa 584-633 / 688. (SwissProt: P10074 Human; SwissProt: Q1H9T6 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBTB48
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZBTB48 Antibody

• GLI-Kruppel family member HKR3
• HKR3zinc finger and BTB domain-containing protein 48
• Krueppel-related zinc finger protein 3
• Protein HKR3
• zinc finger and BTB domain containing 48
• Zinc finger protein 855
• ZNF855pp9964

Background

ZBTB48 binds to and regulates the J and/or S elements in MHC II promoter

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBTB48