XAB2 Antibody

Western Blot: XAB2 Antibody [NB100-794] - NB100-794 staining (0.3ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: XAB2 Antibody [NB100-794] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

XAB2 Antibody Summary

• Description: Novus Biologicals Goat XAB2 Antibody (NB100-794) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-XAB2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SVPAAVFGSLKED corresponding to C-Terminus according to NP_064581.2.
• Localization: Nuclear, detected in the splicing complex carrying pre-mRNA.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: XAB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 4 ug/ml
  • Immunohistochemistry-Paraffin 3 - 4 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human fibroblast A431 lysates (calculated MW of 100 kDa band according to NP_064581.2). IHC-P: Human brain (cerebral cortex) shows nuclear staining.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XAB2 Antibody

• DKFZp762C1015
• HCNPcrn-related protein kim1
• HCRN
• KIAA1177
• NTC90
• Protein HCNP
• SYF1 homolog, RNA splicing factor
• SYF1pre-mRNA-splicing factor SYF1
• XPA binding protein 2
• XPA-binding protein 2

Background

Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XAB2 Antibody (NB100-794)(1)

Publication using NB100-794

• Nakatsu Y, Asahina H, Citterio E et al. XAB2, a novel tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. J Biol Chem 2000-11-10 [PMID: 10944529]

Bioinformatics

• Gene Symbol: XAB2
• Entrez:
  • 56949(Human)
• Uniprot:
  • NP_064581.2(Human)