XAB2 Antibody

Images

 
Immunohistochemistry: XAB2 Antibody [NB100-794] - NB100-794 staining (0.3ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: XAB2 Antibody [NB100-794] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

XAB2 Antibody Summary

Immunogen
Peptide with sequence C-SVPAAVFGSLKED corresponding to C-Terminus according to NP_064581.2.
Localization
Nuclear, detected in the splicing complex carrying pre-mRNA.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
XAB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 3 - 4 ug/ml
  • Immunohistochemistry-Paraffin 3 - 4 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 90 kDa band observed in human fibroblast A431 lysates (calculated MW of 100 kDa band according to NP_064581.2). IHC-P: Human brain (cerebral cortex) shows nuclear staining.
Publications
Read Publication using NB100-794.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XAB2 Antibody

  • DKFZp762C1015
  • HCNPcrn-related protein kim1
  • HCRN
  • KIAA1177
  • NTC90
  • Protein HCNP
  • SYF1 homolog, RNA splicing factor
  • SYF1pre-mRNA-splicing factor SYF1
  • XPA binding protein 2
  • XPA-binding protein 2

Background

Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XAB2 Antibody (NB100-794)(1)

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Bioinformatics

Gene Symbol XAB2
Entrez
Uniprot