WTX Antibody (729419) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 729419
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

WTX Antibody (729419) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human WTX
  Met421-Leu573
  Accession # Q5JTC6
• Specificity: Detects human WTX in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WTX Antibody (729419) [Alexa Fluor® 700]

• AMER1
• FAM123B
• OSCS
• WTX

Background

WTX (Wilms Tumor on the X; also FAM123B and AMER1) is a 190-200 kDa member of the FAM123 family of proteins. It is widely expressed in both adult and embryo and appears to have at least three functions: one, it binds WT1 in the nucleus and regulates cell differentiation; two, it associates with the plasma membrane and draws APC away from microtubules, thereby participating in intercellular junction integrity; and three, it binds beta -catenin and impacts WNT signaling. Human WTX is 1135 amino acids in length. It contains a PtdIns-P2 binding site (aa 2-209), an acidic domain (aa 370-411), and a Pro-rich region (aa 952-1104). There are three APC binding motifs (aa 280-368; 380-431; 717-834) and the beta -catenin binding region lies C-terminal to Gly368. There are two isoform variants. One is 150 kDa in size, nuclear in location, and shows a deletion of aa 50-326. A second shows a 19 aa substitution for aa 786-1135. Over aa 421-573, human WTX shares 89% aa identity with mouse WTX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.