WFS1 Overexpression Lysate (Native)

Images

 
Western Blot: WFS1 Overexpression Lysate (Adult Normal) [NBL1-17845] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WFS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WFS1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202901 and based on accession number NM_006005. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 1, mRNA.
Gene
WFS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
100.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for WFS1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WFS1 Overexpression Lysate (Native)

  • DFNA14
  • DFNA38
  • DFNA6
  • DIDMOAD
  • FLJ51211
  • WFRS
  • WFS1
  • WFSwolframin
  • Wolfram syndrome 1 (wolframin)
  • Wolframin

Background

This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) is the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for WFS1 Lysate (NBL1-17845) (0)

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Video Protocols

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Bioinformatics

Gene Symbol WFS1
Entrez