WFS1 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

WFS1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human WFS1
  Lys679-Phe783
  Accession # O76024
• Specificity: Detects human WFS1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WFS1 Antibody [Alexa Fluor® 647]

• DFNA14
• DFNA38
• DFNA6
• DIDMOAD
• FLJ51211
• WFRS
• WFS1
• WFSwolframin
• Wolfram syndrome 1 (wolframin)
• Wolframin

Background

WFS1 (Wolframin Syndrome gene 1; also Wolframin) is a 100-105 kDa intracellular glycoprotein that contains an unusual eleven transmembrane (TM) topology. It is widely expressed, being found in neurons, fibroblasts, hepatocytes, stratified squamous epithelium and pancreatic beta -cells. WFS1 is found in the ER and select secretory vesicles. It is known to be induced by ER stress, which prompts it to increase Ca⁺⁺ in the ER, a condition necessary for proper protein folding. It also contributes to the maintenance of the proper pH in insulin-containing granules. Human WSF1 is 890 amino acids (aa) in length. It is a type II 11-TM protein that possesses a cytoplasmic N-terminus (aa 1-313) and transmembrane-embedded C-terminus (aa 870-890). WSF1 is reported to form homodimers and homotetramers. There are multiple mutations in the WFS1 gene that contribute to Wolfram syndrome. Among these are an isoform that generates a premature truncation at Ser157, a second isoform that possesses a seven aa substitution for aa 509-890, and a third isoform which shows a deletion of aa 508-512. Over aa 679-783, human WFS1 shares 95% aa sequence identity with mouse WFS1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.