VPS45 Antibody

Western Blot: VPS45 Antibody [NB100-41372] - Staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: VPS45 Antibody [NB100-41372] - Staining of paraffin embedded Human Placenta.Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VPS45 Antibody Summary

• Immunogen: Peptide with sequence C-ESSQVTSRSASRR corresponding to C-Terminus according to NP_009190.2.
• Predicted Species: Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VPS45
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 60 kDa band observed in human testis and peripheral blood mononucleocyte lysates (calculated MW of 65.1 kDa band according to NP_009190.2).
• Control:

  VPS45 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VPS45 Antibody

• H1
• H1VPS45
• hlVps45
• h-vps45
• leucocyte vacuolar protein sorting 45
• vacuolar protein sorting 45 homolog (S. cerevisiae)
• vacuolar protein sorting 45A (yeast homolog)
• vacuolar protein sorting 45A (yeast)
• vacuolar protein sorting 45A
• vacuolar protein sorting-associated protein 45
• VPS45A
• VPS45AVPS54A
• VPS45B
• VSP45
• VSP45A

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS45 Antibody (NB100-41372)(1)

Publication using NB100-41372

• Rajasekariah P, Eyre HJ, Stanley KK et al. Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45). Int J Biochem Cell Biol 1999-06-01 [PMID: 10404641]

Bioinformatics

• Gene Symbol: VPS45
• Entrez:
  • 11311(Human)
  • 22365(Mouse)
• Uniprot:
  • NP_009190.2(Human)