VPS45 Antibody

Images

 
Western Blot: VPS45 Antibody [NB100-41372] - (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VPS45 Antibody Summary

Immunogen
Peptide with sequence C-ESSQVTSRSASRR corresponding to C-Terminus according to NP_009190.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS45
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 60 kDa band observed in human testis and peripheral blood mononucleocyte lysates (calculated MW of 65.1 kDa band according to NP_009190.2).
Positive Control
VPS45 Lysate (NBL1-17753)
Publications
Read Publication using NB100-41372.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS45 Antibody

  • H1
  • H1VPS45
  • hlVps45
  • h-vps45
  • leucocyte vacuolar protein sorting 45
  • vacuolar protein sorting 45 homolog (S. cerevisiae)
  • vacuolar protein sorting 45A (yeast homolog)
  • vacuolar protein sorting 45A (yeast)
  • vacuolar protein sorting 45A
  • vacuolar protein sorting-associated protein 45
  • VPS45A
  • VPS45AVPS54A
  • VPS45B
  • VSP45
  • VSP45A

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS45 Antibody (NB100-41372)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS45
Entrez
Uniprot