VISTA/B7-H5/PD-1H Antibody (730804) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71261) or (B) Mouse IgG2BIsotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P). View our protocol for Staining Membrane-associated Proteins.

VISTA/B7-H5/PD-1H was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB71261) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 730804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VISTA/B7-H5/PD-1H Antibody (730804) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VISTA/B7-H5/PD-1H
  Phe33-Ala194
  Accession # Q9H7M9
• Specificity: Detects human VISTA/B7-H5/PD-1H in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse VISTA/B7-H5/PD-1H is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: C10ORF54
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VISTA/B7-H5/PD-1H Antibody (730804) [Unconjugated]

• 4632428N05Rik
• B7H5
• B7-H5
• C10orf54
• chromosome 10 open reading frame 54
• Dies1
• Gi24
• PD1H
• PD-1H
• platelet receptor Gi24
• PP2135
• SISP1
• stress induced secreted protein 1
• VISTA
• VSIR

Background

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VISTA/B7-H5/PD-1H Antibody (MAB71261)(2)

Publications using MAB71261

• R Liu, J Merola, TD Manes, L Qin, GT Tietjen, F López-Girá, V Broecker, C Fang, C Xie, PM Chen, NC Kirkiles-S, D Jane-Wit, JS Pober Interferon-? converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1 JCI Insight, 2018-03-08;3(5):. 2018-03-08 [PMID: 29515027] (Flow Cytometry, Human)
• Bharaj , Preeti, Chahar , Harendra, Alozie , Ogechika, Rodarte , Lizette, Bansal , Anju, Goepfert , Paul A, Dwivedi , Alok, Manjunath , N, Shankar , Premlata Characterization of programmed death-1 homologue-1 (PD-1H) expression and function in normal and HIV infected individuals. PLoS ONE, 2014-10-03;9(10):e109103. 2014-10-03 [PMID: 25279955] (Flow Cytometry, IHC, Human)

Bioinformatics

• Gene Symbol: C10ORF54
• Uniprot:
  • Q9H7M9(Human)