visinin-like 1 Antibody - BSA Free

Western Blot: visinin-like 1 Antibody [NBP2-25168] - Analysis of different tissue lysates using chicken pAb to visinin-like protein 1 (VLP1), NBP2-25168, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at ~20kDa corresponds to VLP1 protein.

Immunohistochemistry: visinin-like 1 Antibody [NBP2-25168] - Confocal image of adult rat cerebellar cortex stained with NBP2-25168 (green), polyclonal antibody to NF-M: NB300-133 (red) and DNA (blue). NBP2-25168 reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) and dendrites and axons are revealed with NF-M antibody. Little staining of visinin-like 1 is seen in the white matter (WM).

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

visinin-like 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken visinin-like 1 Antibody - BSA Free (NBP2-25168) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length recombinant human VLP1
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: VSNL1
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:1000-1:2000
  • Western Blot 1:5000-1:10000
• Application Notes: This visinin-like 1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~22 kDa.
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
• Preservative: 0.02% Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for visinin-like 1 Antibody - BSA Free

• HLP3Hippocalcin-like protein 3
• HPCAL3
• HUVISL1
• VILIP
• VILIP-1
• VILIPVLP-1
• visinin-like 1
• visinin-like protein 1
• VISL1
• VSNL1

Background

Visinin was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VSNL1
• Entrez:
  • 7447(Human)
• OMIM:
  • 600817(Human)
• Uniprot:
  • P62760(Human)