VIGR/GPR126 Antibody (1044430) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human VIGR/GPR126 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human VIGR/GPR126 Monoclonal Antibody (Catalog # MAB10577) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Gate was set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 1044430
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VIGR/GPR126 Antibody (1044430) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived human VIGR/GPR126 protein
  Cys38-Lys437
  Accession # AAH75798.1
• Specificity: Detects human VIGR/GPR126 protein in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VIGR/GPR126 Antibody (1044430) [Unconjugated]

• APG1
• Developmentally regulated G-protein-coupled receptor
• DREG
• FLJ14937
• G protein-coupled receptor 126
• Gm222
• GPR126
• G-protein coupled receptor 126
• HBV PreS1-transactivated protein 2
• PS1TP2
• Vascular inducible G protein-coupled receptor
• vascular-inducible G protein-coupled receptor
• VIGR
• VIGRPS1TP2

Background

VIGR (Vascular Inducible G Protein-coupled Receptor), also known as ADGRG6, DREG, and GPR126, is a neuronal 7 TM pass (G protein)-coupled receptor (GPCR) involved in myelination and glial and Schwann cell development (1, 2). Human VIGR cDNA encodes a 1221 amino acid (aa) residue membrane protein with a 37 aa signal peptide, a 825 aa extracellular domain (ECD) with 27 potential N-linked glycosylation sites, seven transmembrane segments that span between aa 863 and aa 1113, and a 108 aa residue cytoplasmic domain. Within ECD human VIGR shares 83% aa sequence identity with mouse and rat VIGR. VIGR is essential for the development of diverse organs (1, 2). Type IV collagen, a major constituent of the basement membrane, binds to VIGR and activates its signaling function (3). This interaction stimulated the production of cAMP in rodent Schwann cells, which require VIGR activity to differentiate, and in human embryonic kidney (HEK293) cells expressing exogenous VIGR. Laminin-211 binds a novel laminin-binding domain in VIGR N-terminal fragment between aa 446 and 807 (4). VIGR-Laminin-211 interactions regulate terminal differentiation and myelination by ensuring appropriate levels of cAMP for a given stage of Schwann cell development (4).

1. Rughetti, A. et al. (2005) J. Immunol. 174:7764.
2. Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
3. Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
4. Geng, Y. et al. (2012) Front Oncol. 2:76.
5. Tanida, S. et al. (2013) J Biol Chem. 288:31842.
6. Beatson, R. et al. (2016) Nat Immunol. 17:1273.
7. Piyush, T. et al. (2017) Cell Death Differ. 24:1937.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • AAH75798.1()