VIGR/GPR126 Antibody (1044430) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 1044430
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

VIGR/GPR126 Antibody (1044430) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human VIGR/GPR126 protein in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VIGR/GPR126 Antibody (1044430) [Alexa Fluor™ Plus 594]

• APG1
• Developmentally regulated G-protein-coupled receptor
• DREG
• FLJ14937
• G protein-coupled receptor 126
• Gm222
• GPR126
• G-protein coupled receptor 126
• HBV PreS1-transactivated protein 2
• PS1TP2
• Vascular inducible G protein-coupled receptor
• vascular-inducible G protein-coupled receptor
• VIGR
• VIGRPS1TP2

Background

VIGR (Vascular Inducible G Protein-coupled Receptor), also known as ADGRG6, DREG, and GPR126, is a neuronal 7 TM pass (G protein)-coupled receptor (GPCR) involved in myelination and glial and Schwann cell development (1, 2). Human VIGR cDNA encodes a 1221 amino acid (aa) residue membrane protein with a 37 aa signal peptide, a 825 aa extracellular domain (ECD) with 27 potential N-linked glycosylation sites, seven transmembrane segments that span between aa 863 and aa 1113, and a 108 aa residue cytoplasmic domain. Within ECD human VIGR shares 83% aa sequence identity with mouse and rat VIGR. VIGR is essential for the development of diverse organs (1, 2). Type IV collagen, a major constituent of the basement membrane, binds to VIGR and activates its signaling function (3). This interaction stimulated the production of cAMP in rodent Schwann cells, which require VIGR activity to differentiate, and in human embryonic kidney (HEK293) cells expressing exogenous VIGR. Laminin-211 binds a novel laminin-binding domain in VIGR N-terminal fragment between aa 446 and 807 (4). VIGR-Laminin-211 interactions regulate terminal differentiation and myelination by ensuring appropriate levels of cAMP for a given stage of Schwann cell development (4).

1. Rughetti, A. et al. (2005) J. Immunol. 174:7764.
2. Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
3. Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
4. Geng, Y. et al. (2012) Front Oncol. 2:76.
5. Tanida, S. et al. (2013) J Biol Chem. 288:31842.
6. Beatson, R. et al. (2016) Nat Immunol. 17:1273.
7. Piyush, T. et al. (2017) Cell Death Differ. 24:1937.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.