VARS Antibody - BSA Free

Western Blot: VARS Antibody [NBP2-20843] - Sample (30 ug of whole cell lysate) A: Hep G2 B: HCT116 5% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: VARS Antibody [NBP2-20843] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry: VARS Antibody [NBP2-20843] - VARS antibody [N1N2], N-term detects VARS protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VARS antibody [N1N2], N-term (NBP2-20843) dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VARS Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit VARS Antibody - BSA Free (NBP2-20843) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-VARS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human VARS. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VARS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for VARS Antibody - BSA Free

• EC 6.1.1
• EC 6.1.1.9
• G7AVARS1
• Protein G7a
• valine tRNA ligase 1, cytoplasmic
• Valine--tRNA ligase
• ValRS
• valyl-tRNA synthetase 2
• valyl-tRNA synthetase
• VARS2valRS

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for VARS Antibody (NBP2-20843)(1)

Publication using NBP2-20843

• Najla El-Hachem, Marine Leclercq, Miguel Susaeta Ruiz, Raphael Vanleyssem, Kateryna Shostak, Pierre-René Körner, Coralie Capron, Lorena Martin-Morales, Patrick Roncarati, Arnaud Lavergne, Arnaud Blomme, Silvia Turchetto, Eric Goffin, Palaniraja Thandapani, Ivan Tarassov, Laurent Nguyen, Bernard Pirotte, Alain Chariot, Jean-Christophe Marine, Michael Herfs, Francesca Rapino, Reuven Agami, Pierre Close Valine aminoacyl-tRNA synthetase promotes therapy resistance in melanoma. Nature cell biology 2024-06-07 [PMID: 38849541]

Bioinformatics

• Gene Symbol: VARS1
• Uniprot:
  • P26640(Human)