VARS Antibody

Images

 
Western Blot: VARS Antibody [NBP2-20843] - Sample (30 ug of whole cell lysate) A: Hep G2 B: HCT116 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: VARS Antibody [NBP2-20843] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: VARS Antibody [NBP2-20843] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

VARS Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Valyl tRNA synthetase. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VARS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (84%), Rat (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VARS Antibody

  • EC 6.1.1
  • EC 6.1.1.9
  • G7AVARS1
  • Protein G7a
  • valine tRNA ligase 1, cytoplasmic
  • Valine--tRNA ligase
  • ValRS
  • valyl-tRNA synthetase 2
  • valyl-tRNA synthetase
  • VARS2valRS

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol VARS
Uniprot