Vanilloid R1/TRPV1 Antibody

Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NB300-122] - Staining of mouse inferior olive using Cy3-conjugated donkey anti-guinea pig secondary antibody (red color) and DAPI (blue) as a nuclear counterstain.

Immunohistochemistry: Vanilloid R1/TRPV1 Antibody [NB300-122] - Dorsal Root Ganglion

Immunohistochemistry-Frozen: Vanilloid R1/TRPV1 Antibody [NB300-122] - TRPV1 Antibody staining of rat dorsal horn (dilution 1:100).

Product Discontinued

Vanilloid R1/TRPV1 Antibody Summary

• Immunogen: Synthetic peptide (YTGSLKPEDAEVFKDSMVPGEK) corresponding to residues aa 817-836 of the carboxy-terminus of rat TPVR1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Guinea Pig
• Gene: TRPV1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:200
• Application Notes: IHC-Frozen, ICC/IF reactivity reported (PMID: 22998799).

Reactivity Notes

Mouse reactivity per customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Vanilloid R1/TRPV1 Antibody

• Capsaicin Receptor
• Osm-9-like TRP channel 1
• OTRPC1
• transient receptor potential cation channel subfamily V member 1
• transient receptor potential cation channel, subfamily V, member 1
• transient receptor potential vanilloid 1a
• transient receptor potential vanilloid 1b
• TRPV1
• Vanilloid R1
• Vanilloid receptor 1
• vanilloid receptor subtype 1
• VanilloidR1
• VR1
• VR1DKFZp434K0220

Background

VR1 (vanilloid receptor 1) is an ion channel activated by capsaicin, the pungent ingredient in hot peppers, and by noxious thermal heat. It is expressed by and isolated from nociceptive sensory neurons. Sequence analysis suggests that VR1 has transmembrane domains and intracellular N- and C-termini. It shares structural similarities with the family of putative store-operated calcium channels. It is thought to function as a transducer of noxious thermal stimuli and have involvement in inflammatory pain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vanilloid R1/TRPV1 Antibody (NB300-122)(7)

Publications using NB300-122

• Sun JX, Zhu KY, Wang YM et al. Activation of TRPV1 receptor facilitates myelin repair following demyelination via the regulation of microglial function Acta pharmacologica Sinica 2022-10-13 [PMID: 36229601] (IHC-P, Human)
• Kim BE, Hui-Beckman J, Lyubchenko T et al. Transient receptor potential vanilloid 1 plays a major role in low temperature-mediated skin barrier dysfunction The Journal of allergy and clinical immunology 2022-02-19 [PMID: 35189126] (ICC/IF, Human)
• Kishimoto S, Katagiri A, Oyamaguchi A et al. Enhanced Ocular Surface and Intraoral Nociception via a Transient Receptor Potential Vanilloid 1 Mechanism in a Rat Model of Obstructive Sleep Apnea Neuroscience 2021-12-06 [PMID: 34883200] (Rat)
• Lee SH, Tonello R, Im ST et al. Resolvin D3 controls mouse and human nociceptive functions and preclinical progression of psoriasis Theranostics 2020-11-18 [PMID: 33204332]
• Han RT, Kim HB, Kim YB et al. Oxytocin produces thermal analgesia via vasopressin-1a receptor by modulating TRPV1 and potassium conductance in the dorsal root ganglion neurons Korean J. Physiol. Pharmacol. 2018-03-01 [PMID: 29520170] (ICC/IF, Rat)
• Lebovitz EE, Keller JM, Kominsky H et al. Positive allosteric modulation of TRPV1 as a novel analgesic mechanism Mol Pain 2012-09-21 [PMID: 22998799] (IHC-Fr, ICC/IF, Rat)
• Zsombok A, Gao H, Miyata K et al. Immunohistochemical localization of transient receptor potential vanilloid type 1 and insulin receptor substrate 2 and their co-localization with liver-related neurons in the hypothalamus and brainstem. Brain Res;1398:30-9. 2011-06-29 [PMID: 21620379]

Review for Vanilloid R1/TRPV1 Antibody (NB300-122) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Temugin Berta ICC Mouse 03/10/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Dorsal root ganglia
• Species: Mouse
• Lot: 401615

Comments

• Comments: Blocking and antibody solution: BSA1%/Triton 0.2% in PBS
  Dilution TRPV1 antibody: 1/500
  Secondary antibody: Alexa 488

FAQs for Vanilloid R1/TRPV1 Antibody (NB300-122). (Showing 1 - 1 of 1 FAQs).

1. For the Vanilloid R1/TRPV1 antibody...does it bind intracellulary or extracellularly
   • This AB binds a.a region 817-826, which is a cytoplasmic domain according to uniprot:https://www.uniprot.org/uniprot/O35433#subcellular_location

Bioinformatics

• Gene Symbol: TRPV1
• Entrez:
  • 7442(Human)
  • 83810(Rat)
• Uniprot:
  • O35433()