VAMP-1/VAMP-2 Antibody (540524) [Unconjugated]

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse VAMP-1/VAMP-2 Monoclonal Antibody (Catalog # MAB5958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-1/VAMP-2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 540524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VAMP-1/VAMP-2 Antibody (540524) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human VAMP-1
  Met1-Lys96
  Accession # P23763
• Specificity: Detects human and mouse VAMP-1/VAMP-2 in Western blots. This antibody does not react with recombinant VAMP family members 5, 7, and 8.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAMP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAMP-1/VAMP-2 Antibody (540524) [Unconjugated]

• SPAX1
• SYB1
• VAMP1
• VAMP-1
• VAMP-1/VAMP-2
• vesicle associated membrane protein 1

Background

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1‑96) and a 22 aa transmembrane domain (aa 97‑118). There is one coiled-coil region between aa 33‑93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1‑96, human VAMP-1 is 98% aa identical to mouse VAMP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for VAMP-1/VAMP-2 Antibody (MAB5958) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Drosophila.

reviewed by: Steve Stowers ICC Drosophila 02/10/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Larval neuromuscular junction,Larval ventral nerve cord
• Species: Drosophila
• Lot: CCWM0111801A

Comments

• Comments: Antibody recognizes Drosophila homolog of VAMP1/2 (neuronal-synaptobrevin) and gives strong staining.

Bioinformatics

• Gene Symbol: VAMP1
• Uniprot:
  • P23763()