USP34 Antibody (2E2)

Western Blot: USP34 Antibody (2E2) [H00009736-M01] - Antibody reactive against recombinant protein, detection against immunogen (36.74 KDa) .

Immunocytochemistry/Immunofluorescence: USP34 Antibody (2E2) [H00009736-M01] - Analysis of monoclonal antibody to USP34 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: USP34 Antibody (2E2) [H00009736-M01] - Analysis of monoclonal antibody to USP34 on formalin-fixed paraffin-embedded human seminoma. Antibody concentration 3 ug/ml.

ELISA: USP34 Antibody (2E2) [H00009736-M01] - Detection limit for recombinant GST tagged USP34 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

USP34 Antibody (2E2) Summary

• Immunogen: USP34 (NP_055524, 3296 a.a. ~ 3395 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CKEFKDLHCSKDSTLAEEESEFPSTSISAVLSDLADLRSCDGQALPSQDPEVALSLSCGHSRGLFSHMQQHDILDTLCRTIESTIHVVTRISGKGNQAAS
• Specificity: USP34 - ubiquitin specific protease 34
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP34
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP34 Antibody (2E2)

• Deubiquitinating enzyme 34
• EC 3.1.2.15
• EC 3.4.19.12
• FLJ43910
• KIAA0570
• KIAA0570MGC104459
• KIAA0729
• KIAA0729ubiquitin carboxyl-terminal hydrolase 34
• ubiquitin specific peptidase 34
• ubiquitin specific protease 34
• ubiquitin thioesterase 34
• Ubiquitin thiolesterase 34
• Ubiquitin-specific-processing protease 34
• USP34

Background

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP34 Antibody (H00009736-M01)(2)

Publications using H00009736-M01

• Sy SM, Jiang J, O WS et al. The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks. Nucleic Acids Res. 2013-07-17 [PMID: 23863847]
• Bonuccelli G, Casimiro MC, Sotgia F et al. Caveolin-1 (P132L), a Common Breast Cancer Mutation, Confers Mammary Cell Invasiveness and Defines a Novel Stem Cell/Metastasis-Associated Gene Signature. Am J Pathol. 2009-05-01 [PMID: 19395651]

Bioinformatics

• Gene Symbol: USP34
• Entrez:
  • 9736(Human)
• Uniprot:
  • NP_055524(Human)