UNC84B Antibody (JG39-52)

Western Blot: UNC84B Antibody (JG39-52) [NBP2-75696] - Analysis of SUN2 on rat kidney tissue lysate using anti-SUN2 antibody at 1/2,000 dilution.

Immunocytochemistry/Immunofluorescence: UNC84B Antibody (JG39-52) [NBP2-75696] - Staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UNC84B Antibody (JG39-52) [NBP2-75696] - Analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin.

Flow Cytometry: UNC84B Antibody (JG39-52) [NBP2-75696] - Analysis of SK-Br-3 cells with SUN2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: UNC84B Antibody (JG39-52) [NBP2-75696] - Analysis of paraffin-embedded human tonsil tissue using anti-SUN2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UNC84B Antibody (JG39-52) [NBP2-75696] - Analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UNC84B Antibody (JG39-52) [NBP2-75696] - Analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: UNC84B Antibody (JG39-52) [NBP2-75696] - Analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin.

Product Discontinued

UNC84B Antibody (JG39-52) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human UNC84B aa 591-717 / 717. (SwissProt: Q9UH99 Human; SwissProt: Q8BJS4 Mouse)
• Localization: Endosome. Nucleus inner membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SUN2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for UNC84B Antibody (JG39-52)

• FRIGG
• KIAA0668
• MGC133055
• MGC133056
• nuclear envelope protein
• Protein unc-84 homolog B
• Rab5-interacting protein
• rab5IP
• Sad1 and UNC84 domain containing 2
• Sad1 unc-84 domain protein 2
• Sad1/unc-84 protein-like 2
• SUN domain-containing protein 2
• unc-84 homolog B (C. elegans)
• unc-84 homolog B
• UNC84B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUN2