UNC13A/Munc13-1 Antibody [Unconjugated]

Western blot shows lysates of human brain (hippocampus) tissue and mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse UNC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6214) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UNC13A at approximately 190-210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Product Discontinued

UNC13A/Munc13-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human UNC13A
  Asp1525-Gly1698
  Accession # Q9UPW8
• Specificity: Detects human and mouse UNC13A in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: UNC13A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC13A/Munc13-1 Antibody [Unconjugated]

• KIAA1032Munc13-1protein unc-13 homolog A
• Munc13-1
• unc-13 homolog A (C. elegans)
• UNC13A

Background

UNC13A (UNCoordinated 13A; also Munc13-1) is a 190‑210 kDa member of the UNC-13 family, C2 domain protein superfamily of molecules. It is expressed at synaptic junctions, particularly those involved in GABA, acetylcholine and glutamine transmission, and appears to be necessary for neurotransmitter release. In the presynaptic region, UNC13A likely interacts with syntaxin 1, converting it from a closed to an open configuration, thus allowing for SNARE complex formation. Human UNC13A is 1703 amino acids (aa) in length. It contains one C2 domain that mediates dimerization (aa 1‑79), a coiled‑coil region (aa 320‑357), one DAG Zn‑finger region (aa 553‑603), a second C2 domain (aa 663‑769), two MHD domains (aa 1093‑1512) and a third C2 domain (aa 1532‑1637). Western blots of mouse UNC13A show 105 kDa and 70 kDa forms. Over aa 1525‑1698, human UNC13A shares 98% aa identity with mouse UNC13A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UNC13A
• Uniprot:
  • Q9UPW8(Human)