UBR5/EDD Antibody - BSA Free

Western Blot: UBR5/EDD Antibody [NBP3-12948] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with UBR5/EDD antibody (NBP3-12948) diluted at 1:500.

EDD antibody detects EDD protein at nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EDD protein stained by EDD antibody (NBP3-12948) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UBR5/EDD Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human UBR5/EDD. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBR5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 309 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for UBR5/EDD Antibody - BSA Free

• DD5
• E3 ubiquitin protein ligase, HECT domain containing, 1
• E3 ubiquitin-protein ligase, HECT domain-containing 1
• EC 6.3.2
• EC 6.3.2.-
• EDD
• EDD1
• EDD1E3 ubiquitin-protein ligase UBR5
• EDDE3 identified by differential display
• hHYD
• HYD
• HYDFLJ11310
• Hyperplastic discs protein homolog
• KIAA0896MGC57263
• progestin induced protein
• Progestin-induced protein
• ubiquitin protein ligase E3 component n-recognin 5
• ubiquitin-protein ligase
• UBR5

Background

EDD encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBR5