UBR5/EDD Antibody (JE38-53)

Western Blot: UBR5/EDD Antibody (JE38-53) [NBP3-32291] - Western blot analysis of UBR5/EDD on different lysates with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: A549 cell lysate Lane 5: HEK-293 cell lysate Lane 6: LNCaP cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32291) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: UBR5/EDD Antibody (JE38-53) [NBP3-32291] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32291) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: UBR5/EDD Antibody (JE38-53) [NBP3-32291] - Immunocytochemistry analysis of SH-SY5Y cells labeling UBR5/EDD with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UBR5/EDD antibody (NBP3-32291) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: JE38-53
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

UBR5/EDD Antibody (JE38-53) Summary

• Description: Novus Biologicals Rabbit UBR5/EDD Antibody (JE38-53) (NBP3-32291) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human UBR5/EDD aa 1-50 / 2,799. (Uniprot: O95071)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBR5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000
• Theoretical MW: 309 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for UBR5/EDD Antibody (JE38-53)

• DD5
• E3 ubiquitin protein ligase, HECT domain containing, 1
• E3 ubiquitin-protein ligase, HECT domain-containing 1
• EC 6.3.2
• EC 6.3.2.-
• EDD
• EDD1
• EDD1E3 ubiquitin-protein ligase UBR5
• EDDE3 identified by differential display
• hHYD
• HYD
• HYDFLJ11310
• Hyperplastic discs protein homolog
• KIAA0896MGC57263
• progestin induced protein
• Progestin-induced protein
• ubiquitin protein ligase E3 component n-recognin 5
• ubiquitin-protein ligase
• UBR5

Background

EDD encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBR5