UBN1 Antibody

Western Blot: UBN1 Antibody [NBP2-26142] - (1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ze
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

UBN1 Antibody Summary

• Description: Novus Biologicals Goat UBN1 Antibody (NBP2-26142) is a polyclonal antibody validated for use in WB and ELISA. Anti-UBN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DNSEAYDELVPASLT corresponding to internal region according to NP_058632.2.
• Specificity: Reported variants represent identical protein: NP_058632.2, NP_001072982.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Zebrafish (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UBN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 120 kDa band observed in MOLT4 lysates (calculated MW of 122 kDa band according to NP_058632.2). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBN1 Antibody

• HIRA-binding protein
• ubinuclein 1
• ubinuclein-1
• VT
• VT4

Background

UBN1 acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication-i

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBN1 Antibody (NBP2-26142)(1)

Publication using NBP2-26142

• Gruffat H, Lupo J, Morand P et al. The nuclear and adherent junction complex component protein ubinuclein negatively regulates the productive cycle of Epstein-Barr virus in epithelial cells. J Virol 2011-01-01 [PMID: 21084479]

Bioinformatics

• Gene Symbol: UBN1
• Entrez:
  • 29855(Human)
  • 170644(Mouse)
  • 302935(Rat)