Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - UBA1 was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 0.5 uL NBP3-05789 and 60 ug of Immunomagnetic beads Protein G. Primary antibody: NBP3-05789, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 111 kDa Observed band size: 111 kDa

• Reactivity: Hu
• Applications: ELISA, IP
• Clone: 05
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ubiquitin-activating Enzyme/UBE1 (Accession#: NP_003325.2; Ser2-Arg1058).
• Specificity: No cross-reactivity in ELISA with:
  
  Insect cell lysate
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 0.2-1 ul/mg of lysate

Reactivity Notes

No cross-reactivity in ELISA with:

Insect cell lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS (pH 7.0)
• Preservative: 10% Trehalose
• Purity: Protein A purified

Alternate Names for Ubiquitin-activating Enzyme/UBE1 Antibody (05) - Azide and BSA Free

• A1S9T and BN75 temperature sensitivity complementing
• A1S9T
• A1ST
• AMCX1
• GXP1
• MGC4781
• POC20 centriolar protein homolog
• POC20
• Protein A1S9
• SMAX2
• UBA1
• UBA1, ubiquitin-activating enzyme E1 homolog A
• UBA1A
• UBE1
• UBE1A1S9
• UBE1X
• ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
• Ubiquitin-activating enzyme E1
• Ubiquitinactivating Enzyme
• Ubiquitin-activating Enzyme
• ubiquitin-like modifier activating enzyme 1
• ubiquitin-like modifier-activating enzyme 1

Background

E1 Ubiquitin Activating Enzyme is encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA1