UBE2N/Ubc13 Antibody

Western Blot: UBE2N/Ubc13 Antibody [NB100-56357] - Detection of UBE2N/Ubc13 in human heart lysate with anti-UBE2N pcAb this antibody.

Product Discontinued

UBE2N/Ubc13 Antibody Summary

• Immunogen: This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acids 2-19 and 131-148 of human UBE2N.
• Specificity: UBE2N
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2N
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2 ug/ml
• Application Notes: Use in ELISA reported in scientific literature (PMID: 25790189).
• Control:

  Human Heart Whole Tissue Lysate (Adult Whole Normal)
  UBE2N/Ubc13 Overexpression Lysate

Reactivity Notes

Cross reacts with Human. Mouse reactivity reported in scientific literature (PMID: 28195158).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for UBE2N/Ubc13 Antibody

• bendless-like ubiquitin conjugating enzyme
• Bendless-like ubiquitin-conjugating enzyme
• BLU
• EC 6.3.2.19
• MGC131857
• MGC8489
• UBC13
• UbcH13
• UbcH-ben
• UBE2N
• Ubiquitin carrier protein N
• ubiquitin-conjugating enzyme E2 N
• ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)
• ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• Ubiquitin-protein ligase N
• yeast UBC13 homolog

Background

UBE2N (ubiquitin-conjugating enzyme E2N) is a novel human ubiquitin-conjugating enzyme with an ability to form a thiol ester linkage with ubiquitin in an ubiquitin-activating enzyme E1-dependent manner. The amino acid sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. UBE2N protein may be involved in protein degradation mainly in the muscles and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2N/Ubc13 Antibody (NB100-56357)(3)

Publications using NB100-56357

• Shaw DK, Wang X, Brown LJ et al. Infection-derived lipids elicit an immune deficiency circuit in arthropods. Nat Commun. 2017-02-14 [PMID: 28195158]
• Shaw D, Wang X, Brown L et al. Infection-derived lipids elicit an immune deficiency circuit in arthropods. Nature Communications. [PMID: 25790189] (ELISA)
• Ewart-Toland A, Briassouli P, de Koning JP et al. Identification of Stk6/STK15 as a candidate low-penetrance tumor-susceptibility gene in mouse and human. Nat Genet. 2003-08-01 [PMID: 12881723]

Review for UBE2N/Ubc13 Antibody (NB100-56357) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/30/2016

Summary

• Application: Western Blot
• Sample Tested: 293 whole cell lysate
• Species: Human
• Lot: 120401A-02

Bioinformatics

• Gene Symbol: UBE2N
• Entrez:
  • 7334(Human)
• Uniprot:
  • P61088()