UBE2N/Ubc13 Antibody

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Western Blot: UBE2N/Ubc13 Antibody [NB100-56357] - Detection of UBE2N/Ubc13 in human heart lysate with anti-UBE2N pcAb this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

UBE2N/Ubc13 Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides containing amino acids 2-19 and 131-148 of human UBE2N.
Specificity
UBE2N
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2N
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunoprecipitation 1:10-1:500
Control
Heart Lysate (NB820-59217)
UBE2N/Ubc13 Lysate (NBL1-17541)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56357 in the following applications:

Publications
Read Publications using NB100-56357.

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for UBE2N/Ubc13 Antibody

  • bendless-like ubiquitin conjugating enzyme
  • Bendless-like ubiquitin-conjugating enzyme
  • BLU
  • EC 6.3.2.19
  • MGC131857
  • MGC8489
  • UBC13
  • UbcH13
  • UbcH-ben
  • UBE2N
  • Ubiquitin carrier protein N
  • ubiquitin-conjugating enzyme E2 N
  • ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)
  • ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
  • Ubiquitin-protein ligase N
  • yeast UBC13 homolog

Background

UBE2N (ubiquitin-conjugating enzyme E2N) is a novel human ubiquitin-conjugating enzyme with an ability to form a thiol ester linkage with ubiquitin in an ubiquitin-activating enzyme E1-dependent manner. The amino acid sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. UBE2N protein may be involved in protein degradation mainly in the muscles and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBE2N/Ubc13 Antibody (NB100-56357)(3)

Review for UBE2N/Ubc13 Antibody (NB100-56357) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56357:
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(1)
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Images Ratings Applications Species Date Details
Western Blot UBE2N/Ubc13 NB100-56357
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reviewed by:
Anonymous
WB Human 08/30/2016
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Summary

ApplicationWestern Blot
Sample Tested293 whole cell lysate
SpeciesHuman
Lot120401A-02

Blocking

Blocking Details5% skim milk in TBS

Details

Detection Noteswith ECL solution

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FAQs for UBE2N/Ubc13 Antibody (NB100-56357) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for UBE2N/Ubc13 Antibody (NB100-56357)

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Diseases for UBE2N/Ubc13 Antibody (NB100-56357)

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PTMs for UBE2N/Ubc13 Antibody (NB100-56357)

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Recent Reviews

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5
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4
1
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2
0
1
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Anonymous
08/30/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol UBE2N
Entrez
Uniprot