UBE2I/Ubc9 Antibody (SC0534)

Western Blot: UBE2I/Ubc9 Antibody (SC0534) [NBP2-67564] - Western blot analysis of UBE2I/Ubc9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Ant

Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody (SC0534) [NBP2-67564] - Staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody (SC0534) [NBP2-67564] - Analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin.

Western Blot: UBE2I/Ubc9 Antibody (SC0534) [NBP2-67564] - Analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: HUVEC

Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody (SC0534) [NBP2-67564] - Staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody (SC0534) [NBP2-67564] - Analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin.

Product Discontinued

UBE2I/Ubc9 Antibody (SC0534) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human UBE2I/Ubc9 aa 15-60 / 305. (SwissProt: P63279 Human; SwissProt: P63281 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBE2I
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500- 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for UBE2I/Ubc9 Antibody (SC0534)

• C358B7.1
• EC 6.3.2
• EC 6.3.2.-
• p18
• SUMO-1-protein ligase
• SUMO-protein Ligase
• Ubc9
• UBC9SUMO-conjugating enzyme UBC9
• UBCE9
• UBE2I
• Ubiquitin carrier protein 9
• Ubiquitin carrier protein I
• ubiquitin conjugating enzyme 9
• Ubiquitin-conjugating enzyme E2 I
• ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• ubiquitin-conjugating enzyme UbcE2A
• ubiquitin-like protein SUMO-1 conjugating enzyme
• ubiquitin-protein ligase E2I
• Ubiquitin-protein ligase I

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBE2I