UbcH8/Ube2L6 Antibody (k1H3) - BSA Free

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Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized ...read more
Immunocytochemistry/ Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - ICC/IF analysis of UBE2L6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UBE2L6 antibody ...read more
Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 for 2 hours ...read more
Flow Cytometry: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Flow cytometry analysis of UBE2L6 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor ...read more
Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Cell lysates of MCF7 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 . ...read more
Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
k1H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

UbcH8/Ube2L6 Antibody (k1H3) - BSA Free Summary

Immunogen
Recombinant human UbcH8/Ube2L6 (1-152aa) purified from E. coli
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UBE2L6
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for UbcH8/Ube2L6 Antibody (k1H3) - BSA Free

  • EC 6.3.2.19
  • retinoic acid induced gene B protein
  • Retinoic acid-induced gene B protein
  • RIG-B
  • UbcH8
  • UBCH8MGC40331
  • UBE2L6
  • Ubiquitin carrier protein L6
  • ubiquitin/ISG15-conjugating enzyme E2 L6
  • ubiquitin-conjugating enzyme E2L 6
  • Ubiquitin-protein ligase L6

Background

Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for UbcH8/Ube2L6 Antibody (NBP1-04351)

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Bioinformatics

Gene Symbol UBE2L6
Uniprot