Recombinant Human UbcH7/UBE2L3 Protein

Product Discontinued

Recombinant Human UbcH7/UBE2L3 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-154 of Human UbcH7/UBE2L3
  
  

  Source: E.coli

  Amino Acid Sequence: MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2L3
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50 mM HEPES buffer (pH7.5) 150 mM NaCl, 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human UbcH7/UBE2L3 Protein

• E2-F1
• EC 6.3.2.19
• L-UBC
• UBCE7
• UbcH7
• UBCH7UbcM4
• UbcM4
• UBE2L3
• Ubiquitin carrier protein L3
• ubiquitin-conjugating enzyme E2 L3
• Ubiquitin-conjugating enzyme E2-F1
• ubiquitin-conjugating enzyme E2L 3
• ubiquitin-conjugating enzyme UBCH7
• Ubiquitin-protein ligase L3

Background

Human Ubquitin-conjugating enzyme 7(UbcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes(E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human UBE2L1-UBE2L4 gene could potentially encode different isoforms of the UbcH7. UBE2L3 gene, located at chromosome 22q11.2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of UbcH7. UbcH7(154amino acid) was over-expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2L3