Recombinant Human UbcH6/UBE2E1 Protein

Product Discontinued

Recombinant Human UbcH6/UBE2E1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-193 of Human UbcH6/UBE2E1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSDDDSR ASTSSSSSSS SNQQTEKETN TPKKKESKVS MSKNSKLLST SAKRIQKELA DITLDPPPNC SAGPKGDNIY EWRSTILGPP GSVYEGGVFF LDITFTPEYP FKPPKVTFRT RIYHCNINSQ GVICLDILKD NWSPALTISK VLLSICSLLT DCNPADPLVG SIATQYMTNR AEHDRMARQW TKRYAT

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2E1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human UbcH6/UBE2E1 Protein

• EC 6.3.2.19
• UbcH6
• UBE2E1
• Ubiquitin carrier protein E1
• ubiquitin-conjugating enzyme E2 E1
• ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5)
• ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast)
• Ubiquitin-protein ligase E1

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2E1 is a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Recombinant human UBE2E1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2E1