UAP56 Overexpression Lysate (Native)

Images

 
Western Blot: UAP56 Overexpression Lysate (Adult Normal) [NBL1-07917] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UAP56.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

UAP56 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201847 and based on accession number NM_080598. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens HLA-B associated transcript 1 (BAT1), transcript variant 2, mRNA.
Gene
DDX39B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for UAP56

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UAP56 Overexpression Lysate (Native)

  • 56 kDa U2AF65-associated protein
  • ATP-dependent RNA helicase p47
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
  • DEAD box protein UAP56
  • EC 3.6.4.13
  • HLA-B associated transcript 1
  • HLA-B-associated transcript 1 protein
  • spliceosome RNA helicase BAT1
  • UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Background

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternative splice site in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for UAP56 Lysate (NBL1-07917) (0)

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Bioinformatics

Gene Symbol DDX39B