TXNDC Antibody (OTI3H5) - Azide and BSA Free

Western Blot: TXNDC Antibody (OTI3H5) - Azide and BSA Free [NBP2-74725] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TXNDC.

Immunocytochemistry/Immunofluorescence: TXNDC Antibody (OTI3H5) - Azide and BSA Free [NBP2-74725] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TMNDC.

Immunohistochemistry: TXNDC Antibody (OTI3H5) - Azide and BSA Free [NBP2-74725] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: TXNDC Antibody (OTI3H5) - Azide and BSA Free [NBP2-74725] - Analysis of extracts (35ug) from 9 different cell lines by using TMX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Dilution: 1:400

Immunohistochemistry: TXNDC Antibody (OTI3H5) - Azide and BSA Free [NBP2-74725] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TXNDC Antibody (OTI3H5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human TMX1(NP_110382) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 31.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TXNDC Antibody (OTI3H5) - Azide and BSA Free

• DKFZp564E1962
• PDIA11
• protein disulfide isomerase family A, member 11
• thioredoxin domain containing 1
• Thioredoxin domain-containing protein 1
• thioredoxin domain-containing
• thioredoxin-related transmembrane protein 1
• TMXTXNDC
• Transmembrane Trx-related protein
• TXNDC1thioredoxin domain containing

Background

TXNDC may participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMX1