Two pore calcium channel protein 2 Antibody

Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-45234] - (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-45234] - (3.75ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Two pore calcium channel protein 2 Antibody Summary

• Description: Novus Biologicals Goat Two pore calcium channel protein 2 Antibody (NBP1-45234) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Two pore calcium channel protein 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EDELTERLSQHPH corresponding to C-Terminus according to NP_620714.2.
• Epitope: C-Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPCN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 ug/ml
• Application Notes: WB: Preliminary experiments gave an approx. 30 kDa band in human brain (cerebral cortex), kidney and liver lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 85.2 kDa band according to NP_620714.2. The 30 kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Pancreas and Colon.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Two pore calcium channel protein 2 Antibody

• FLJ41094
• SHEP10
• TPC2
• TPC2two pore calcium channel protein 2
• TPCN2
• Two pore calcium channel protein 2
• two pore segment channel 2
• two-pore calcium channel protein 2
• Voltage-dependent calcium channel protein TPC2

Background

Function: May function as one of the major voltage-gated Ca2+ channel (VDCC) across the plasma membrane.; Subcellular location: Membrane; Multi-pass membrane protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Two pore calcium channel protein 2 Antibody (NBP1-45234)(1)

Publication using NBP1-45234

• Calcraft PJ, Ruas M, Pan Z et al. NAADP mobilizes calcium from acidic organelles through two-pore channels. Nature 2009-05-01 [PMID: 19387438]

Bioinformatics

• Gene Symbol: TPCN2
• Entrez:
  • 219931(Human)
• Uniprot:
  • NP_620714.2(Human)