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TTC30B Overexpression Lysate

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Western Blot: TTC30B Overexpression Lysate (Adult Normal) [NBL1-17406] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TTC30B.
Western Blot: TTC30B Overexpression Lysate (Adult Normal) [NBL1-17406] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TTC30B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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TTC30B Overexpression Lysate Summary

Description

TTC30B Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207665

Accession#: NM_152517

Protein Tag: C-MYC/DDK
Gene
TTC30B

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
75.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TTC30B Overexpression Lysate

  • FLJ30990
  • tetratricopeptide repeat domain 30B
  • tetratricopeptide repeat protein 30B
  • TPR repeat protein 30B

Background

TTC30B is required for polyglutamylation of axonemal tubulin . Plays a role in anterogradeintraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium tothe site of their incorporation at the tip

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.
NF-M Antibody
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Bioinformatics

Gene Symbol TTC30B