TSPAN32/TSSC6 Antibody

Western Blot: TSPAN32/TSSC6 Antibody [NB100-1035] - (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TSPAN32/TSSC6 Antibody Summary

• Immunogen: Peptide with sequence GGLSGCPERGLSD corresponding to C-Terminus according to NP_620591.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TSPAN32
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 33-35 kDa band observed in HeLa lysate.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TSPAN32/TSSC6 Antibody

• ART1
• FLJ17158
• FLJ97586
• MGC22455
• pan-hematopoietic expression
• PHEMX
• PHEMXPHMX
• PHMX
• Protein Phemx
• tetraspanin 32
• tetraspanin-32
• TSPAN32
• tspan-32
• TSSC6
• TSSC6pan-hematopoietic expression protein
• tumor-suppressing STF cDNA 6
• tumor-suppressing subchromosomal transferable fragment cDNA 6
• tumor-suppressing subtransferable candidate 6

Background

This gene is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4 (TSSC4), escapes imprinting. This gene may play a role in malignancies and disease that involve this region as well as hematopoietic cell function. This gene is described as a member of the tetraspanin superfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSPAN32/TSSC6 Antibody (NB100-1035)(1)

Publication using NB100-1035

• Lee MP, Brandenburg S, Landes GM et al. Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting. Hum Mol Genet 1999 Apr [PMID: 10072438]

Bioinformatics

• Gene Symbol: TSPAN32
• Entrez:
  • 10077(Human)