TSC2 Antibody (614204) [Unconjugated]

Images

 
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell ...read more
TSC2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) at 3 µg/mL for 3 hours at room temperature. Cells were ...read more
TSC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse TSC2 Monoclonal Antibody (Catalog # MAB40401) at 15 µg/mL overnight at 4 °C. Before incubation with the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
614204
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

TSC2 Antibody (614204) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TSC2
Val550-Leu850
Accession # P49815
Specificity
Detects human and mouse TSC2 in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TSC2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSC2 Antibody (614204) [Unconjugated]

  • LAM
  • LAMFLJ43106
  • TSC2
  • TSC4
  • Tuberin
  • Tuberous sclerosis 2 protein
  • tuberous sclerosis 2

Background

TSC2, also called tuberin, complexes with TSC1 (hamartin) to inhibit cell proliferation by antagonizing mTOR/p70S6K signaling. Its location either in the cytoplasm or the nucleus is regulated by phosphorylation and the cell cycle. Numerous point mutations are associated with development of tumors (hamartomas) in kidney, heart, skin and brain. Human TSC2 shares 91% amino acid (aa) identity with mouse TSC2 over aa 550-850.  Splice variants that are missing aa 946 to 988 or 989 may show different tissue distribution than full length TSC2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Blogs on TSC2. Showing 1-10 of 49 blog posts - Show all blog posts.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

TSC2 - GTPase activating protein involved in cell cycle inhibition
TSC2 is a tumor suppressor gene that encodes a 200 kDa protein called tuberin. TSC2 heterodimerizes with TSC1 to form a complex with GTPase-activating protein (GAP) activity. The C-terminus of TSC2 contains the GAP domain responsible for this cata...  Read full blog post.

Showing 1-10 of 49 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol TSC2
Entrez
Uniprot