Tryptase alpha/TPS1 Antibody (AA1)

Images

 
Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Human tonsil stained with Mast Cell Tryptase antibody.
Immunohistochemistry-Paraffin: Tryptase alpha/TPS1 Antibody (AA1) [NBP2-26444] - Analysis of Mast Cell Tryptase formalin-fixed paraffin embedded human tonsil using NBP2-26444.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
AA1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Tryptase alpha/TPS1 Antibody (AA1) Summary

Immunogen
Purified human mast cell tryptase was used as immunogen (Walls et al, 1990).
Localization
Cytoplasmic
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TPSAB1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
Publications
Read Publications using
NBP2-26444 in the following applications:

  • IHC
    2 publications
  • 8 publications
  • WB
    1 publication

Reactivity Notes

Rat and mouse reactivity reported in scientific literature. Please see Publications tab for full list. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Tryptase alpha/TPS1 Antibody (AA1)

  • alpha-Tryptase
  • TPS1
  • TPS2
  • TPSAB1
  • TPSB1
  • Tryptase 1
  • Tryptase alpha
  • tryptase alpha/beta 1

Background

Principal Names: Tryptase alpha/beta 1 Official Gene Symbol: TPSAB1 Gene ID: 7177 (Human) Gene Map Locus: 16p13.3 (Human). Human tryptases are divided into two groups, alpha and beta. beta-Tryptase appears to be the major type stored in mast cell secretory granules and alpha-tryptase is the major isoform expressed by basophils.beta-tryptase, a major constituent of mast cell secretory granules, has emerged recently as the most specific marker in vivo of mast cell activation in humans. It is a trypsin like serine protease that hydrolyzes peptide bonds on the C-terminal side of basic amino acids. Unlike trypsin, however, it is highly selective in hydrolyzing its peptide and protein targets and resists inactivation by circulating inhibitors. It has potential roles in the lung, skin, and other tissues as a local anticoagulant, activator of collagenase, promoter of smooth muscle contraction, and modulator of neuropeptide activity. The pathogenic role for tryptases has been observed in asthmatic airway inflammation other inflammatory disorders. The gene is mapped on human chromosome 16p13.3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tryptase alpha/TPS1 Antibody (NBP2-26444)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(8)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(6)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-26444 Applications Species
Gao F, Fish BL, Szabo A et al. Enhanced survival from radiation pneumonitis by combined irradiation to the skin. Int. J. Radiat. Biol. 2014 May 14 [PMID: 24827855] (IHC-P, Rat) IHC-P Rat
Farkas D, Kraskauskas D, Drake JI et al. CXCR4 inhibition ameliorates severe obliterative pulmonary hypertension and accumulation of C-kit(+) cells in rats. PLoS ONE. 2014 Mar 3 [PMID: 24587052] (IHC-P, Rat) IHC-P Rat
Masaka T, Iijima K, Endo H et al. Gender differences in oesophageal mucosal injury in a reflux oesophagitis model of rats. Gut. 2013 Jan [PMID: 22287598] (IHC-P, Rat)

Details:
Product cited: Mast Cell Tryptase (IMG-80250). IHC(paraffin): Normal and reflux esophagitis (RE) rat esophagus tissue: Fig S1.
IHC-P Rat
Le BV, Khorsi-Cauet H, Bach V, Gay-Queheillard J. Mast cells mediate Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation in rat. Eur J Clin Microbiol Infect Dis. 2012 Aug [PMID: 22282020]

Details:
Products cited: Mast Cell Tryptase (IMG-80250).
Shimada R, Nakao K, Furutani R, Kibayashi K. A rat model of changes in dural mast cells and brain histamine receptor H3 expression following traumatic brain injury. J Clin Neurosci. 2012 Mar [PMID: 22277566] (IHC-P, Mouse)

Details:
Product Cited: Mast Cell Tryptase (IMG-80250). 1. IHC(paraffin): Mouse brain (Cerebral Cortex) with/out induced traumatic brain injury (TBI), Figure 1A & B.
IHC-P Mouse
Sato Y, Harada K, Kizawa K et al. Activation of the MEK5/ERK5 cascade is responsible for biliary dysgenesis in a rat model of Caroli's disease. Am J Pathol. 2005 Jan [PMID: 15631999] (IHC-P, Rat)

Details:
IHC (paraffin): rat liver, Fig 3B inset.
IHC-P Rat
Haase MG, Klawitter A, Baretton GB. IkappaBgamma is expressed in mast cells. Virchows Arch. 2004 Nov [PMID: 15378359] (IHC-P, Rat)

Details:
IHC (paraffin): Fig 1 (rat lung tissue sections), Fig 2 (rat peritoneal macrophages).
IHC-P Rat
Akiyoshi H, Terada T. Mast cell, myofibroblast and nerve terminal complexes in carbon tetrachloride-induced cirrhotic rat livers. J Hepatol. 1998 Jul [PMID: 9696499] (IHC-P, Rat)

Details:
IHC (paraffin): rat liver (data described in Results section, page 114).
IHC-P Rat
Walls AF, Jones DB, Williams JH et al. Immunohistochemical identification of mast cells in formaldehyde-fixed tissue using monoclonal antibodies specific for tryptase. J Pathol. 1990 Oct [PMID: 2250189] (WB, IHC-P, Human)

Details:
IHC (paraffin) in human: Fig 1 (skin), Fig 2 (lung), Fig 4 (A:bronchiole, B:lung alveoli, C:T-lymphocyte area of tonsil, D:septum of tonsil, E:colonic mucosa and F:skin), Table 1 (summary of staining in multiple tissues). Peripheral blood basophils were u
WB, IHC-P Human
Maiese A, Scopetti M, Santurro A et al. Corpse dismemberment: A case series. Solving the puzzle through an integrated multidisciplinary approach Journal of Forensic and Legal Medicine Jan 1 2020 (IHC, Human) IHC Human
Show All 11 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TPSAB1